TRIM32 Antibody (2E5) - Azide and BSA Free

Novus Biologicals | Catalog # H00022954-M09

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2E5

Format

Azide and BSA Free
View all TRIM32 Primary Antibodies »

Product Specifications

Immunogen

TRIM32 (NP_036342, 105 a.a. ~ 204 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RRLPRQFCRSCGLVLCEPCREADHQPPGHCTLPVKEAAEERRRDFGEKLTRLRELMGELQRRKAALEGVSKDLQARYKAVLQEYGHEERRVQDELARSRK

Specificity

TRIM32 (2E5)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for TRIM32 Antibody (2E5) - Azide and BSA Free

Western Blot: TRIM32 Antibody (2E5) [H00022954-M09]

Western Blot: TRIM32 Antibody (2E5) [H00022954-M09]

Western Blot: TRIM32 Antibody (2E5) [H00022954-M09] - Analysis of TRIM32 expression in transfected 293T cell line by TRIM32 monoclonal antibody (M09), clone 2E5. Lane 1: TRIM32 transfected lysatE (72 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: TRIM32 Antibody (2E5) [H00022954-M09]

Immunocytochemistry/ Immunofluorescence: TRIM32 Antibody (2E5) [H00022954-M09]

Immunocytochemistry/Immunofluorescence: TRIM32 Antibody (2E5) [H00022954-M09] - Analysis of monoclonal antibody to TRIM32 on HeLa cell. Antibody concentration 10 ug/ml
Western Blot: TRIM32 Antibody (2E5) [H00022954-M09]

Western Blot: TRIM32 Antibody (2E5) [H00022954-M09]

Western Blot: TRIM32 Antibody (2E5) [H00022954-M09] - Analysis of TRIM32 over-expressed 293 cell line, cotransfected with TRIM32 Validated Chimera RNAi or non-transfected control. Blot probed with H00022954-M09. GAPDH (36.1 kDa) used as loading control.
ELISA: TRIM32 Antibody (2E5) [H00022954-M09]

ELISA: TRIM32 Antibody (2E5) [H00022954-M09]

ELISA: TRIM32 Antibody (2E5) [H00022954-M09] - Detection limit for recombinant GST tagged TRIM32 is approximately 0.3ng/ml as a capture antibody.

Applications for TRIM32 Antibody (2E5) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein for western blot. It has also been used for RNAi validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TRIM32

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes.

Long Name

Tripartite Motif-containing Protein 32

Alternate Names

BBS11, HT2A, LGMD2H, TATIP

Entrez Gene IDs

22954 (Human)

Gene Symbol

TRIM32

OMIM

254110 (Human)

UniProt

NP_036342 (Human)

Additional TRIM32 Products

Product Documents for TRIM32 Antibody (2E5) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for TRIM32 Antibody (2E5) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TRIM32 Antibody (2E5) - Azide and BSA Free

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Protocols

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FAQs

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