Triosephosphate isomerase Antibody (181) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-43761

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Predicted:

Bovine (100%), Canine (100%), Chimpanzee (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # 181

Format

Azide and BSA Free
View all Triosephosphate isomerase Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Triosephosphate isomerase. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Theoretical MW

31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Triosephosphate isomerase Antibody (181) - Azide and BSA Free

Western Blot: Triosephosphate isomerase Antibody (181) [NBP2-43761]

Western Blot: Triosephosphate isomerase Antibody (181) [NBP2-43761]

Western Blot: Triosephosphate isomerase Antibody (181) [NBP2-43761] - Analysis of A. 30 ug 293T whole cell extract B. 30 ug A431 whole cell extract C. 30 ug HeLa whole cell extract D. 30 ug HepG2 whole cell extract 12 % SDS-PAGE Triosephosphate isomerase antibody [181] dilution: 1:1000
Western Blot: Triosephosphate isomerase Antibody (181) [NBP2-43761]

Western Blot: Triosephosphate isomerase Antibody (181) [NBP2-43761]

Western Blot: Triosephosphate isomerase Antibody (181) [NBP2-43761] - Analysis of A. 30 ug Neuro2A whole cell extract B. 30 ug C8D30 whole cell extract C. 30 ug NIH-3T3 whole cell extract D. 30 ug Raw264.7 whole cell extract E. 30 ug C2C12 whole cell extract 12 % SDS-PAGE Triosephosphate isomerase antibody [181] dilution: 1:1000
Western Blot: Triosephosphate isomerase Antibody (181) [NBP2-43761]

Western Blot: Triosephosphate isomerase Antibody (181) [NBP2-43761]

Western Blot: Triosephosphate isomerase Antibody (181) [NBP2-43761] - Analysis of A. 30 ug PC-12 whole cell extract B. 30 ug Rat2 whole cell extract 12 % SDS-PAGE Triosephosphate isomerase antibody [181] dilution: 1:1000
Triosephosphate isomerase Antibody (181) - Azide and BSA Free

Immunohistochemistry-Paraffin: Triosephosphate isomerase Antibody (181) [NBP2-43761] -

Triosephosphate isomerase antibody [GT181] detects Triosephosphate isomerase protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Triosephosphate isomerase stained by Triosephosphate isomerase antibody [GT181] (NBP2-43761) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Triosephosphate isomerase Antibody (181) - Azide and BSA Free

Western Blot: Triosephosphate isomerase Antibody (181) [NBP2-43761] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Triosephosphate isomerase antibody [GT181] (NBP2-43761) diluted at 1:500.
Triosephosphate isomerase Antibody (181) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Triosephosphate isomerase Antibody (181) [NBP2-43761] -

Triosephosphate isomerase antibody [GT181] detects Triosephosphate isomerase protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Triosephosphate isomerase protein stained by Triosephosphate isomerase antibody [GT181] (NBP2-43761) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Applications for Triosephosphate isomerase Antibody (181) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Triosephosphate isomerase

Triosephosphate isomerase (TIM) catalyses the reversible interconversion of G3P and DHAP. Only G3P can be used in glycolysis, therefore TIM is essential for energy production, allowing two molecules of G3P to be produced for every glucose molecule, thereby doubling the energy yield. Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency) [MIM:190450]. TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection.

Alternate Names

EC 5.3.1.1, MGC88108, TIM, TPI, triosephosphate isomerase, Triose-phosphate isomerase, triosephosphate isomerase 1

Gene Symbol

TPI1

Additional Triosephosphate isomerase Products

Product Documents for Triosephosphate isomerase Antibody (181) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Triosephosphate isomerase Antibody (181) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Triosephosphate isomerase Antibody (181) - Azide and BSA Free

Customer Reviews for Triosephosphate isomerase Antibody (181) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review Triosephosphate isomerase Antibody (181) - Azide and BSA Free and earn rewards!

Have you used Triosephosphate isomerase Antibody (181) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies