TrkA Antibody (MNAC13) - Chimeric - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-81074

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, ELISA, Block/Neutralize, Immunocytochemistry/ Immunofluorescence, Surface Plasmon Resonance

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # MNAC13

Format

Azide and BSA Free
View all TrkA Primary Antibodies »

Product Specifications

Immunogen

Human TrkA ECD.

Specificity

This antibody binds to the extracellular domain of TrkA. It was shown not to cross react with TrkB. Moreover MNAC13 is specific to TrkA with negligible reactivity to TrkB and TrkC.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for TrkA Antibody (MNAC13) - Chimeric - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: TrkA Antibody (MNAC13) - Chimeric - Azide and BSA Free [NBP2-81074]

Immunocytochemistry/ Immunofluorescence: TrkA Antibody (MNAC13) - Chimeric - Azide and BSA Free [NBP2-81074]

Immunocytochemistry/Immunofluorescence: TrkA Antibody (MNAC13) [NBP2-81074] - Immunofluoresence staining of fixed HeLa cells with anti-TrkA antibody (MNAC13) [NBP2-81074]. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of MNAC13 [NBP2-81074] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81074, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.

Applications for TrkA Antibody (MNAC13) - Chimeric - Azide and BSA Free

Application
Recommended Usage

Block/Neutralize

Optimal dilutions of this antibody should be experimentally determined.

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Surface Plasmon Resonance

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. This antibody binds to human TrkA, a receptor tyrosine kinase involved in the development and the maturation of the CNS and the PNS through regulation of proliferation, differentiation, and survival of the sympathetic and nervous neurons. It is a receptor for NGF and phosphorylates several downstream proteins regulating a variety of signalling cascades. This antibody neutralizes the NGF-TrkA interation.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: TrkA

The Trk proto-oncogene encodes a 140 kDa, membrane-spanning protein tyrosine kinase that is expressed only in neural tissues. Nerve growth factor (NGF) stimulates phosphorylation of Trk A in neural cell lines and in embryonic dorsal root ganglia. Affinity cross-linking and equilibrium binding experiments with 125I-labeled NGF indicate that Trk A binds NGF specifically in cultured cells with a dissociation constant of 10(-9) molar. The identification of Trk A as an NGF receptor indicates that this protein participates in the primary signal transduction mechanism of NGF (1). Trk A was found to be expressed in the nervous system and phosphorylated in response to NGF (Nerve Growth Factor). Somatic rearrangement(s) of the TRKA gene (also designated NTRK1) are responsible for formation of some oncogenes (2). Trk A is expressed in neural and nonneuronal tissues. Like RET, Trk A is often activated by rearrangements that involve one of at least five other genes in papillary thyroid carcinoma (PTC) (3).

Long Name

Neurotrophic Tyrosine Kinase Receptor A

Alternate Names

NTRK-1, NTRK1

Gene Symbol

NTRK1

Additional TrkA Products

Product Documents for TrkA Antibody (MNAC13) - Chimeric - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for TrkA Antibody (MNAC13) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Receptor Tyrosine Kinases (RTKs)

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Protocols

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FAQs for TrkA Antibody (MNAC13) - Chimeric - Azide and BSA Free

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  • Q: I am searching (with difficulty) for an antibody against Trka which can be used in flow cytometry. Since I am planning to use it for bead sorting of live cells it needs to be for a part of the receptor which is on the outside of the cell. Please can you let me know if you can help me with this.

    A:

    NBP1-47436, NB100-98815 and NB100-65266 all bind to the extracellular domain of Trka, however, none of them are guaranteed for use in flow cytometry. Our only flow cytometry guaranteed antibodies bind to the intracellular domain. This does not mean one of these antibodies won't work for flow, we just can not guarantee it. If you would be interested in testing this novel application on one of our Trka antibodies, please take a look at our Innovators Reward Program.

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