TrkB Antibody (10B6C4) - BSA Free

ELISA: TrkB Antibody (10B6C4) - BSA Free [NBP2-52524]

ELISA: TrkB Antibody (10B6C4) [NBP2-52524] - lack line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Western Blot: TrkB Antibody (10B6C4) - BSA Free [NBP2-52524] -

TrkB expression in human brain and platelets. (A) Healthy human cortex (C; 3 ug) and platelets lysates (platelets; 30 to 75 ug) were analyzed in denaturing and reducing conditions and blotted with antibodies raised against TrkB extracellular domain. Left: molecular weight marker in kDa. Right: antibody catalog number. beta -actin was used as a loading control. (B) Human glioblastoma cells U87-MG and healthy platelets lysates were either left untreated (Untreated) or submitted to 37C overnight in absence (Sham) or presence of N-deglycosylation enzyme PNGase F (PNGase F). Membranes were blotted with ANT-019 antibody against TrkB ECD. PNGase F activity was confirmed by reblotting membranes with antibodies against N-glycosylated proteins sortilin for U87-MG and CD42b for platelets. (C) Human glioblastoma U87-MG cells and healthy human platelets isolated from whole blood were fixed or fixed and permeabilized. Cells were labeled with antibodies directed toward the extracellular domain of the TrkB receptor and analyzed by flow cytometry. Results shown are representative of (A) ≥ 3 independent experiments and ≥ 5 different platelet samples from different donors and (B, C) 3 independent experiments and 3 different donors for platelets samples. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33643311), licensed under a CC-BY license. Not internally tested by Novus Biologicals.