TrkB Antibody (OTI2E1)

Novus Biologicals | Catalog # NBP1-47898

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2E1
View all TrkB Primary Antibodies »

Product Specifications

Immunogen

Full-length protein expressed in 293T cell transfected with human NTRK2 expression vector

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens neurotrophic tyrosine kinase, receptor, type 2 (NTRK2), transcript variant b.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse TrkB Antibody (OTI2E1) (NBP1-47898) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. Anti-TrkB Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for TrkB Antibody (OTI2E1)

Western Blot: TrkB Antibody (OTI2E1) [NBP1-47898]

Western Blot: TrkB Antibody (OTI2E1) [NBP1-47898]

Western Blot: TrkB Antibody (2E1) [NBP1-47898] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY NTRK2 (RC205129, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antiNTRK2.
Immunocytochemistry/Immunofluorescence: TrkB Antibody (2E1) [NBP1-47898] Staining of COS7 cells transiently transfected by pCMV6-ENTRY TrkB.
Immunohistochemistry-Paraffin: TrkB Antibody (OTI2E1) [NBP1-47898]

Immunohistochemistry-Paraffin: TrkB Antibody (OTI2E1) [NBP1-47898]

Immunohistochemistry-Paraffin: TrkB Antibody (2E1) [NBP1-47898] - Staining of paraffin-embedded Human lung tissue using anti-TrkB mouse monoclonal antibody.

Applications for TrkB Antibody (OTI2E1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1.17 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TrkB

This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in this gene have been associated with obesity and mood disorders. Alternate transcriptional splice variants encoding different isoforms have been found for this gene

Long Name

Neurotrophic Tyrosine Kinase Receptor B

Alternate Names

NTRK2

Entrez Gene IDs

4915 (Human)

Gene Symbol

NTRK2

Additional TrkB Products

Product Documents for TrkB Antibody (OTI2E1)

Certificate of Analysis

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Product Specific Notices for TrkB Antibody (OTI2E1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TrkB Antibody (OTI2E1)

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Protocols

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