Troponin C (cardiac) Antibody (1F8-A9) - Azide and BSA Free

Novus Biologicals | Catalog # H00007134-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1F8-A9

Format

Azide and BSA Free
View all Troponin C (cardiac) Primary Antibodies »

Product Specifications

Immunogen

TNNC1 (AAH30244, 1 a.a. ~ 161 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDDIYKAAVEQLTEEQKNEFKAAFDIFVLGAEDGCISTKELGKVMRMLGQNPTPEELQEMIDEVDEDGSGTVDFDEFLVMMVRCMKDDSKGKSEEELSDLFRMFDKNADGYIDLDELKIMLQATGETITEDDIEELMKDGDKNNDGRIDYDEFLEFMKGVE

Localization

Cytoplasmic

Specificity

TNNC1 - troponin C, slow

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Troponin C (cardiac) Antibody (1F8-A9) - Azide and BSA Free

Western Blot: Troponin C (cardiac) Antibody (1F8-A9) [H00007134-M01]

Western Blot: Troponin C (cardiac) Antibody (1F8-A9) [H00007134-M01]

Western Blot: Troponin C (cardiac) Antibody (1F8-A9) [H00007134-M01] - Analysis of TNNC1 expression in transfected 293T cell line by TNNC1 monoclonal antibody (M01), clone 1F8-A9.Lane 1: TNNC1 transfected lysate(18.4 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: Troponin C (cardiac) Antibody (1F8-A9) [H00007134-M01]

Immunohistochemistry-Paraffin: Troponin C (cardiac) Antibody (1F8-A9) [H00007134-M01]

Immunohistochemistry-Paraffin: Troponin C (cardiac) Antibody (1F8-A9) [H00007134-M01] - Analysis of monoclonal antibody to TNNC1 on formalin-fixed paraffin-embedded human heart. Antibody concentration 3 ug/ml.
ELISA: Troponin C (cardiac) Antibody (1F8-A9) [H00007134-M01]

ELISA: Troponin C (cardiac) Antibody (1F8-A9) [H00007134-M01]

ELISA: Troponin C (cardiac) Antibody (1F8-A9) [H00007134-M01] - Detection limit for recombinant GST tagged TNNC1 is approximately 0.03ng/ml as a capture antibody.

Applications for Troponin C (cardiac) Antibody (1F8-A9) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Troponin C (cardiac)

Troponin I is part of a heteromeric complex playing an important role in the regulation of skeletal and cardiac muscle contraction. It consists of three subunits, troponin I (TnI), troponin T (TnT) and troponin C (TnC). Each subunit is responsible for part of troponin complex function. TnI inhibits ATPase activity of acto myosin and TnT and TnI are present in cardiac muscles in different forms than in skeletal muscles. Only one tissue specific isoform of TnI is described for cardiac muscle tissue (cTnI) and this is expressed only in myocardium.

Alternate Names

cardiac troponin C, CMD1Z, CMH13, slow twitch skeletal/cardiac muscle troponin C, TNC, TN-C, TNNC, troponin C type 1 (slow), troponin C, slow, troponin C, slow skeletal and cardiac muscles, troponin C1, slow

Entrez Gene IDs

7134 (Human)

Gene Symbol

TNNC1

OMIM

191040 (Human)

UniProt

AAH30244 (Human)

Additional Troponin C (cardiac) Products

Product Documents for Troponin C (cardiac) Antibody (1F8-A9) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Troponin C (cardiac) Antibody (1F8-A9) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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