TRPM4 Antibody (CL11214) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-43938

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL11214

Format

Azide and BSA Free
View all TRPM4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of Q8TD43, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for TRPM4 Antibody (CL11214) - Azide and BSA Free

TRPM4 Antibody (CL11214) - Azide and BSA Free Immunohistochemistry-Paraffin: TRPM4 Antibody [NBP3-43938]

Immunohistochemistry-Paraffin: TRPM4 Antibody [NBP3-43938]

Analysis in human duodenum and pancreas tissues using NBP3-43938 antibody. Corresponding TRPM4 RNA-seq data are presented for the same tissues.
TRPM4 Antibody (CL11214) - Azide and BSA Free Immunohistochemistry-Paraffin: TRPM4 Antibody [NBP3-43938]

Immunohistochemistry-Paraffin: TRPM4 Antibody [NBP3-43938]

Staining of human kidney shows moderate to strong membranous positivity in cells in tubules.
TRPM4 Antibody (CL11214) - Azide and BSA Free Immunohistochemistry-Paraffin: TRPM4 Antibody [NBP3-43938]

Immunohistochemistry-Paraffin: TRPM4 Antibody [NBP3-43938]

Staining of human duodenum shows strong membranous positivity in glandular cells.
TRPM4 Antibody (CL11214) - Azide and BSA Free Immunohistochemistry-Paraffin: TRPM4 Antibody [NBP3-43938]

Immunohistochemistry-Paraffin: TRPM4 Antibody [NBP3-43938]

Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
TRPM4 Antibody (CL11214) - Azide and BSA Free Immunohistochemistry-Paraffin: TRPM4 Antibody [NBP3-43938]

Immunohistochemistry-Paraffin: TRPM4 Antibody [NBP3-43938]

Staining of human prostate shows moderate membranous positivity in glandular cells.
TRPM4 Antibody (CL11214) - Azide and BSA Free Western Blot: TRPM4 Antibody [NBP3-43938]

Western Blot: TRPM4 Antibody [NBP3-43938]

Analysis in human cell line RT-4.
TRPM4 Antibody (CL11214) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: TRPM4 Antibody [NBP3-43938]

Immunocytochemistry/ Immunofluorescence: TRPM4 Antibody [NBP3-43938]

Staining of MCF7 cells using the Anti-TRPM4 monoclonal antibody).

Applications for TRPM4 Antibody (CL11214) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1 ug/ml
Application Notes
Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TRPM4

FUNCTION: Calcium-activated non selective (CAN) cation channel that mediates membrane depolarization. While it is activated by increase in intracellular Ca(2+), it is impermeable to it. Mediates transport of monovalent cations (Na(+) > K(+) > Cs(+) > Li(+)), leading to depolarize the membrane. It thereby plays a central role in cadiomyocytes, neurons from entorhinal cortex, dorsal root and vomeronasal neurons, endocrine pancreas cells, kidney epithelial cells, cochlea hair cells etc. Participates in T-cell activation by modulating Ca(2+) oscillations after T lymphocyte activation, which is required for NFAT-dependent IL2 production. Involved in myogenic constriction of cerebral arteries. Controls insulin secretion in pancreatic beta-cells. May also be involved in pacemaking or could cause irregular electrical activity under conditions of Ca(2+) overload.; ENZYME REGULATION: Gating is voltage-dependent and repressed by decavanadate. Calmodulin-binding confers the Ca(2+) sensitivity. ATP is able to restore Ca(2+) sensitivity after desensitization. Phosphatidylinositol 4,5-biphosphate (PIP2)-binding strongly enhances activity, by increasing the channel's Ca(2+) sensitivity and shifting its voltage dependence of activation towards negative potentials. Activity is also enhanced by 3,5-bis(trifluoromethyl)pyrazole derivative (BTP2). SUBUNIT: Homomultimer. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Widely expressed with a high expression in intestine and prostate. In brain, it is both expressed in whole cerebral arteries and isolated vascular smooth muscle cells.

Long Name

Transient receptor potential cation channel subfamily M member 4

Alternate Names

hTRPM4, LTrpC-4, LTrpC4, Melastatin-4

Gene Symbol

TRPM4

Additional TRPM4 Products

Product Documents for TRPM4 Antibody (CL11214) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for TRPM4 Antibody (CL11214) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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