Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07)

Novus Biologicals | Catalog # NBP2-67580

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SC53-07 expressed in HEK293
View all Tryptophan Hydroxylase 1/TPH-1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human Tryptophan Hydroxylase 1/TPH-1 aa 372-419 / 444. (SwissProt: P17752 Human; SwissProt: P17532 Mouse; SwissProt: P09810 Rat)

Localization

Cytosol, neuron projection.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07)

Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580]

Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580]

Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580] - Analysis of Tryptophan Hydroxylase 1/TPH1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HL-60 cell lysate Lane 2: Hela cell lysate
Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580]

Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580]

Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580] - Analysis of paraffin-embedded rat brain tissue using anti-Tryptophan Hydroxylase/TPH-1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Flow Cytometry: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580]

Flow Cytometry: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580]

Flow Cytometry: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580] - Analysis of Hela cells with TPH1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580]

Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580]

Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580] - Analysis of paraffin-embedded mouse cerebellum tissue using anti-TPH1 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580]

Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580]

Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580] - Analysis of paraffin-embedded rat brain tissue using anti-TPH1 antibody. Counter stained with hematoxylin.
Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07)

Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580] -

Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580] - Analysis of TPH1 on different lysates with Rabbit anti-TPH1 antibody at 1/1,000 dilution.Lane 1: MDA-MB-231-si NT cell lysateLane 2: MDA-MB-231-si TPH1 cell lysateLysates/proteins at 10 ug/Lane.Predicted band size: 51 kDaObserved band size: 72 kDaExposure time: 3 minutes;4-20% SDS-PAGE gel.NBP2-67580 was shown to specifically react with TPH1 in MDA-MB-231-si NT cells. Weakened band was observed when MDA-MB-231-si TPH1 sample was tested. MDA-MB-231-si NT and Hela-si TPH1 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (1/1,000) and Loading control antibody (Rabbit anti-GAPDH, (1/10,000) were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-rabbit IgG-HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.

Applications for Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Immunoprecipitation

-Use at an assay dependent concentration

Western Blot

1:500

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Tryptophan Hydroxylase 1/TPH-1

Tryptophan hydroxylase (TPH) is the rate-limiting enzyme in the biosynthesis of serotonin (5-HT) and might be related to the pathogenesis of major depression (MD). Two isoforms are known, TPH1 and TPH2. (1). TPH1 is phosphorylated by cAMP-dependent protein kinase A (2). Recently, it has been shown that expression of the TPH1 gene is increased in lungs and pulmonary endothelial cells from patients with idiopathic pulmonary arterial hypertension. Results indicate that TPH1 and peripheral serotonin play an essential role in the development of hypoxia-induced elevations in pulmonary pressures and hypoxia-induced pulmonary vascular remodeling. In addition, the results suggest that, in mice, serotonin has differential effects on the pulmonary vasculature and right ventricular hypertrophy (3).

Alternate Names

TPH-1, TPRH

Gene Symbol

TPH1

Additional Tryptophan Hydroxylase 1/TPH-1 Products

Product Documents for Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07)

Certificate of Analysis

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Product Specific Notices for Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07)

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Protocols

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