TUFM Antibody (CL2242)
Novus Biologicals | Catalog # NBP3-52054
Recombinant Monoclonal Antibody
![TUFM Antibody (CL2242) Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2242) [NBP3-52054]](https://resources.rndsystems.com/images/products/nbp3-52054_mouse-tufm-mab-cl2242-immunocytochemistry-immunofluorescence-27320261527313.jpg "Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2242) [NBP3-52054]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # CL2242
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Product Specifications
Immunogen
This antibody was developed using a recombinant protein derived from P49411, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for TUFM Antibody (CL2242)
Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2242) [NBP3-52054]
Staining in U251 cell line showing distinct mitochondrial staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).![TUFM Antibody (CL2242) Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2242) [NBP3-52054]](https://resources.rndsystems.com/images/products/nbp3-52054_mouse-tufm-mab-cl2242-immunocytochemistry-immunofluorescence-2732026153005.jpg "Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2242) [NBP3-52054]")
Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2242) [NBP3-52054]
Staining in HeLa cell line showing distinct mitochondrial staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).![TUFM Antibody (CL2242) Western Blot: TUFM Antibody (CL2242) [NBP3-52054]](https://resources.rndsystems.com/images/products/nbp3-52054_mouse-tufm-mab-cl2242-western-blot-27320261519249.jpg "Western Blot: TUFM Antibody (CL2242) [NBP3-52054]")
Western Blot: TUFM Antibody (CL2242) [NBP3-52054]
Lane 1: Marker [kDa]Lane 2: Human cell line HeLa cytoplasmic fraction
Lane 3: Human cell line HeLa membrane fraction
Lane 4: Human cell line HeLa nuclear fraction
Lane 5: Human cell line HeLa chromatin fraction
Lane 6: Human cell line HeLa cytoskeletal fraction
![TUFM Antibody (CL2242) Immunohistochemistry: TUFM Antibody (CL2242) [NBP3-52054]](https://resources.rndsystems.com/images/products/nbp3-52054_mouse-tufm-mab-cl2242-immunohistochemistry-2732026153009.jpg "Immunohistochemistry: TUFM Antibody (CL2242) [NBP3-52054]")
Immunohistochemistry: TUFM Antibody (CL2242) [NBP3-52054]
Staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.![TUFM Antibody (CL2242) Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2242) [NBP3-52054]](https://resources.rndsystems.com/images/products/nbp3-52054_mouse-tufm-mab-cl2242-immunocytochemistry-immunofluorescence-273202615235212.jpg "Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2242) [NBP3-52054]")
Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2242) [NBP3-52054]
Staining in U2OS cell line showing distinct mitochondrial staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).![TUFM Antibody (CL2242) Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2242) [NBP3-52054]](https://resources.rndsystems.com/images/products/nbp3-52054_mouse-tufm-mab-cl2242-immunocytochemistry-immunofluorescence-2732026151967.jpg "Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2242) [NBP3-52054]")
Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2242) [NBP3-52054]
Staining in MCF7 cell line showing distinct mitochondrial staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).![TUFM Antibody (CL2242) Immunohistochemistry: TUFM Antibody (CL2242) [NBP3-52054]](https://resources.rndsystems.com/images/products/nbp3-52054_mouse-tufm-mab-cl2242-immunohistochemistry-273202615284411.jpg "Immunohistochemistry: TUFM Antibody (CL2242) [NBP3-52054]")
Immunohistochemistry: TUFM Antibody (CL2242) [NBP3-52054]
Staining of human fallopian tube shows strong granular cytoplasmic positivity in glandular cells.![TUFM Antibody (CL2242) Western Blot: TUFM Antibody (CL2242) [NBP3-52054]](https://resources.rndsystems.com/images/products/nbp3-52054_mouse-tufm-mab-cl2242-western-blot-27320261520453.jpg "Western Blot: TUFM Antibody (CL2242) [NBP3-52054]")
Western Blot: TUFM Antibody (CL2242) [NBP3-52054]
Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-PPIB.![TUFM Antibody (CL2242) Immunohistochemistry: TUFM Antibody (CL2242) [NBP3-52054]](https://resources.rndsystems.com/images/products/nbp3-52054_mouse-tufm-mab-cl2242-immunohistochemistry-273202615273111.jpg "Immunohistochemistry: TUFM Antibody (CL2242) [NBP3-52054]")
Immunohistochemistry: TUFM Antibody (CL2242) [NBP3-52054]
Staining of human testis shows strong granular cytoplasmic positivity in cells in seminiferous ducts and in Leydig cells.![TUFM Antibody (CL2242) Western Blot: TUFM Antibody (CL2242) [NBP3-52054]](https://resources.rndsystems.com/images/products/nbp3-52054_mouse-tufm-mab-cl2242-western-blot-273202615282014.jpg "Western Blot: TUFM Antibody (CL2242) [NBP3-52054]")
Western Blot: TUFM Antibody (CL2242) [NBP3-52054]
Lane 1: Marker [kDa]Lane 2: Human cell line U-251 MG
![TUFM Antibody (CL2242) Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2242) [NBP3-52054]](https://resources.rndsystems.com/images/products/nbp3-52054_mouse-tufm-mab-cl2242-immunocytochemistry-immunofluorescence-273202615291324.jpg "Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2242) [NBP3-52054]")
Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2242) [NBP3-52054]
Staining in A431 cell line showing distinct mitochondrial staining in green. Microtubule and nuclear probes are visualized in red and blue respectively (where available).![TUFM Antibody (CL2242) Immunohistochemistry: TUFM Antibody (CL2242) [NBP3-52054]](https://resources.rndsystems.com/images/products/nbp3-52054_mouse-tufm-mab-cl2242-immunohistochemistry-273202615222519.jpg "Immunohistochemistry: TUFM Antibody (CL2242) [NBP3-52054]")
Immunohistochemistry: TUFM Antibody (CL2242) [NBP3-52054]
Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules and moderate staining in glomerular cells.Applications for TUFM Antibody (CL2242)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TUFM
Alternate Names
EFTu, EF-Tu, EFTUCOXPD4, EF-TuMT, elongation factor Tu, mitochondrial, P43, Tu translation elongation factor, mitochondrial
Gene Symbol
TUFM
Additional TUFM Products
Product Documents for TUFM Antibody (CL2242)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for TUFM Antibody (CL2242)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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