TUFM Antibody (CL2242)

Novus Biologicals | Catalog # NBP3-52456

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # CL2242
View all TUFM Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P49411, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for TUFM Antibody (CL2242)

Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2242) [NBP3-52456]

Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2242) [NBP3-52456]

Staining in A431 cell line showing distinct mitochondrial staining in green. Microtubule and nuclear probes are visualized in red and blue respectively (where available).
Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2242) [NBP3-52456]

Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2242) [NBP3-52456]

Staining in HeLa cell line showing distinct mitochondrial staining in green. Microtubule and nuclear probes are visualized in red and blue respectively (where available).
Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2242) [NBP3-52456]

Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2242) [NBP3-52456]

Staining in MCF7 cell line showing distinct mitochondrial staining in green. Microtubule and nuclear probes are visualized in red and blue respectively (where available).
Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2242) [NBP3-52456]

Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2242) [NBP3-52456]

Staining in U251 cell line showing distinct mitochondrial staining in green. Microtubule and nuclear probes are visualized in red and blue respectively (where available).
Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2242) [NBP3-52456]

Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2242) [NBP3-52456]

Staining in U2OS cell line showing distinct mitochondrial staining in green. Microtubule and nuclear probes are visualized in red and blue respectively (where available).

Applications for TUFM Antibody (CL2242)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Preservative

0.02% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TUFM

TUFM encodes a protein which participates in protein translation in mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency resulting in lactic acidosis and fatal encephalopathy. A pseudogene has been identified on chromosome 17. [provided by RefSeq]

Alternate Names

EFTu, EF-Tu, EFTUCOXPD4, EF-TuMT, elongation factor Tu, mitochondrial, P43, Tu translation elongation factor, mitochondrial

Gene Symbol

TUFM

Additional TUFM Products

Product Documents for TUFM Antibody (CL2242)

Certificate of Analysis

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Product Specific Notices for TUFM Antibody (CL2242)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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