TWA1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32596

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Rat

Cited:

Human

Predicted:

Bovine (97%), Chicken (97%), Mouse (99%), Rhesus Macaque (100%), Xenopus (96%). Backed by our 100% Guarantee.

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human TWA1. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (96%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TWA1 Antibody - BSA Free

Western Blot: TWA1 Antibody [NBP1-32596]

Western Blot: TWA1 Antibody [NBP1-32596]

TWA1-Antibody-Western-Blot-NBP1-32596-img0006.jpg
Immunocytochemistry/ Immunofluorescence: TWA1 Antibody [NBP1-32596]

Immunocytochemistry/ Immunofluorescence: TWA1 Antibody [NBP1-32596]

Immunocytochemistry/Immunofluorescence: TWA1 Antibody [NBP1-32596] - Paraformaldehyde-fixed HeLa, using antibody at 1:1000 dilution.
Immunohistochemistry-Paraffin: TWA1 Antibody [NBP1-32596]

Immunohistochemistry-Paraffin: TWA1 Antibody [NBP1-32596]

Immunohistochemistry-Paraffin: TWA1 Antibody [NBP1-32596] - Paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.
Western Blot: TWA1 Antibody [NBP1-32596]

Western Blot: TWA1 Antibody [NBP1-32596]

Western Blot: TWA1 Antibody [NBP1-32596] - (30 ug whole cell lysate) A: 293T 12% SDS PAGE; antibody diluted at 1:1000
Knockout Validated: TWA1 Antibody [NBP1-32596]

Western Blot: TWA1 Antibody [NBP1-32596]

TWA1-Antibody-Knockout-Validated-NBP1-32596-img0005.jpg
TWA1 Antibody

Western Blot: TWA1 Antibody [NBP1-32596] -

Western Blot: TWA1 Antibody [NBP1-32596] - RanBPM & TWA1 are essential for complex stability. Whole cell extracts prepared from control shRNA & RanBPM shRNA HEK293 cells (a), or from control (labelled as C), TWA1, RMND5A, MAEA, ARMC8 & muskelin HEK293 CRISPR knockout cells (b–f) were analyzed by Western blot with antibodies to CTLH complex members, as indicated. Vinculin was used as a loading control. Quantifications are shown below each blot & protein levels are shown relative to control cells set to 1 & normalized to Vinculin levels. Data represent averages from three separate experiments, with error bars indicating SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31285494), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
TWA1 Antibody

Western Blot: TWA1 Antibody [NBP1-32596] -

Western Blot: TWA1 Antibody [NBP1-32596] - RanBPM & TWA1 are essential for complex stability. Whole cell extracts prepared from control shRNA & RanBPM shRNA HEK293 cells (a), or from control (labelled as C), TWA1, RMND5A, MAEA, ARMC8 & muskelin HEK293 CRISPR knockout cells (b–f) were analyzed by Western blot with antibodies to CTLH complex members, as indicated. Vinculin was used as a loading control. Quantifications are shown below each blot & protein levels are shown relative to control cells set to 1 & normalized to Vinculin levels. Data represent averages from three separate experiments, with error bars indicating SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31285494), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
TWA1 Antibody

Western Blot: TWA1 Antibody [NBP1-32596] -

Western Blot: TWA1 Antibody [NBP1-32596] - RanBPM & TWA1 are essential for complex stability. Whole cell extracts prepared from control shRNA & RanBPM shRNA HEK293 cells (a), or from control (labelled as C), TWA1, RMND5A, MAEA, ARMC8 & muskelin HEK293 CRISPR knockout cells (b–f) were analyzed by Western blot with antibodies to CTLH complex members, as indicated. Vinculin was used as a loading control. Quantifications are shown below each blot & protein levels are shown relative to control cells set to 1 & normalized to Vinculin levels. Data represent averages from three separate experiments, with error bars indicating SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31285494), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
TWA1 Antibody

Western Blot: TWA1 Antibody [NBP1-32596] -

Western Blot: TWA1 Antibody [NBP1-32596] - RanBPM & TWA1 are essential for complex stability. Whole cell extracts prepared from control shRNA & RanBPM shRNA HEK293 cells (a), or from control (labelled as C), TWA1, RMND5A, MAEA, ARMC8 & muskelin HEK293 CRISPR knockout cells (b–f) were analyzed by Western blot with antibodies to CTLH complex members, as indicated. Vinculin was used as a loading control. Quantifications are shown below each blot & protein levels are shown relative to control cells set to 1 & normalized to Vinculin levels. Data represent averages from three separate experiments, with error bars indicating SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31285494), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for TWA1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

Assay dependent

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TWA1

Alternate Names

bA305P22.1, C20orf11, chromosome 20 open reading frame 11, FLJ20602, GID complex subunit 8 homolog (S. cerevisiae), Twa1, TWA1protein C20orf11, two hybrid associated protein 1, Two hybrid-associated protein 1 with RanBPM

Gene Symbol

GID8

Additional TWA1 Products

Product Documents for TWA1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for TWA1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for TWA1 Antibody - BSA Free

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Protocols

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