U2AF35 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-92553

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all U2AF35 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MHLKPISRELRRELYGRRRKKHRSRS

Reactivity Notes

Reactivity reported in scientific literature (PMID: 24579871)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for U2AF35 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: U2AF35 Antibody [NBP1-92553]

Immunocytochemistry/ Immunofluorescence: U2AF35 Antibody [NBP1-92553]

Immunocytochemistry/Immunofluorescence: U2AF35 Antibody [NBP1-92553] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: U2AF35 Antibody [NBP1-92553]

Immunohistochemistry-Paraffin: U2AF35 Antibody [NBP1-92553]

Immunohistochemistry-Paraffin: U2AF35 Antibody [NBP1-92553] - Staining of human hippocampus shows moderate nuclear positivity in neuronal cells.
U2AF35 Antibody - BSA Free Immunohistochemistry: U2AF35 Antibody - BSA Free [NBP1-92553]

Immunohistochemistry: U2AF35 Antibody - BSA Free [NBP1-92553]

Staining of human duodenum shows strong nuclear positivity in glandular cells.
U2AF35 Antibody - BSA Free Immunohistochemistry: U2AF35 Antibody - BSA Free [NBP1-92553]

Immunohistochemistry: U2AF35 Antibody - BSA Free [NBP1-92553]

Staining of human cerebral cortex shows strong nuclear positivity in neurons.
U2AF35 Antibody - BSA Free Immunohistochemistry: U2AF35 Antibody - BSA Free [NBP1-92553]

Immunohistochemistry: U2AF35 Antibody - BSA Free [NBP1-92553]

Staining of human skin shows strong nuclear positivity in squamous epithelial cells.
U2AF35 Antibody - BSA Free Immunohistochemistry: U2AF35 Antibody - BSA Free [NBP1-92553]

Immunohistochemistry: U2AF35 Antibody - BSA Free [NBP1-92553]

Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
U2AF35 Antibody - BSA Free Western Blot: U2AF35 Antibody - BSA Free [NBP1-92553]

Western Blot: U2AF35 Antibody - BSA Free [NBP1-92553]

Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.

Applications for U2AF35 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: U2AF35

U2AF35 belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Alternate Names

FP793, human U2 snRNP auxiliary factor small subunit10U2AF35DKFZp313J1712, RN, RNU2AF1,35-KD subunit, U2 auxiliary factor 35 kDa subunit, U2 small nuclear RNA auxiliary factor 1, U2 small nuclear RNA auxillary factor 1, U2 snRNP auxiliary factor small subunit

Gene Symbol

U2AF1

Additional U2AF35 Products

Product Documents for U2AF35 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for U2AF35 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for U2AF35 Antibody - BSA Free

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Protocols

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FAQs

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