UbcH10/UBE2C Antibody (9D3) - Azide and BSA Free

Novus Biologicals | Catalog # H00011065-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 9D3

Format

Azide and BSA Free
View all UbcH10/UBE2C Primary Antibodies »

Product Specifications

Immunogen

UBE2C (NP_008950, 70 a.a. ~ 179 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AGTVYEDLRYKLSLEFPSGYPYNAPTVKFLTPCYHPNVDTQGNICLDILKEKWSALYDVRTILLSIQSLLGEPNIDSPLNTHAAELWKNPTAFKKYLQETYSKQVTSQEP

Localization

Nuclear

Specificity

UBE2C - ubiquitin-conjugating enzyme E2C

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for UbcH10/UBE2C Antibody (9D3) - Azide and BSA Free

Western Blot: UbcH10/UBE2C Antibody (9D3) [H00011065-M01]

Western Blot: UbcH10/UBE2C Antibody (9D3) [H00011065-M01]

Western Blot: UbcH10/UBE2C Antibody (9D3) [H00011065-M01] - UBE2C monoclonal antibody (M01), clone 9D3 Analysis of UBE2C expression in HeLa.
Immunocytochemistry/ Immunofluorescence: UbcH10/UBE2C Antibody (9D3) [H00011065-M01]

Immunocytochemistry/ Immunofluorescence: UbcH10/UBE2C Antibody (9D3) [H00011065-M01]

Immunocytochemistry/Immunofluorescence: UbcH10/UBE2C Antibody (9D3) [H00011065-M01] - Analysis of monoclonal antibody to UBE2C on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: UbcH10/UBE2C Antibody (9D3) [H00011065-M01]

Immunohistochemistry-Paraffin: UbcH10/UBE2C Antibody (9D3) [H00011065-M01]

Immunohistochemistry-Paraffin: UbcH10/UBE2C Antibody (9D3) [H00011065-M01] - Analysis of monoclonal antibody to UBE2C on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.
Western Blot: UbcH10/UBE2C Antibody (9D3) [H00011065-M01]

Western Blot: UbcH10/UBE2C Antibody (9D3) [H00011065-M01]

Western Blot: UbcH10/UBE2C Antibody (9D3) [H00011065-M01] - Analysis of UBE2C expression in transfected 293T cell line by UBE2C monoclonal antibody (M01), clone 9D3.Lane 1: UBE2C transfected lysate(19.7 KDa).Lane 2: Non-transfected lysate.
ELISA: UbcH10/UBE2C Antibody (9D3) [H00011065-M01]

ELISA: UbcH10/UBE2C Antibody (9D3) [H00011065-M01]

ELISA: UbcH10/UBE2C Antibody (9D3) [H00011065-M01] - Detection limit for recombinant GST tagged UBE2C is approximately 0.03ng/ml as a capture antibody.

Applications for UbcH10/UBE2C Antibody (9D3) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: UbcH10/UBE2C

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined.

Alternate Names

UBE2C

Entrez Gene IDs

11065 (Human)

Gene Symbol

UBE2C

OMIM

605574 (Human)

UniProt

NP_008950 (Human)

Additional UbcH10/UBE2C Products

Product Documents for UbcH10/UBE2C Antibody (9D3) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for UbcH10/UBE2C Antibody (9D3) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for UbcH10/UBE2C Antibody (9D3) - Azide and BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

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Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail