UbcH8/Ube2L6 Antibody (k1H3) - BSA Free

Novus Biologicals | Catalog # NBP1-04351

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # k1H3

Format

BSA Free
View all UbcH8 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human UbcH8/Ube2L6 (1-152aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for UbcH8/Ube2L6 Antibody (k1H3) - BSA Free

Western Blot: UbcH8/Ube2L6 Antibody (k1H3)BSA Free [NBP1-04351]

Western Blot: UbcH8/Ube2L6 Antibody (k1H3)BSA Free [NBP1-04351]

Western Blot: UbcH8/Ube2L6 Antibody (k1H3) [NBP1-04351] - The Cell lysate (20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human UBE2L6 antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Jurkat cell lysate
Immunocytochemistry/ Immunofluorescence: UbcH8/Ube2L6 Antibody (k1H3) - BSA Free [NBP1-04351]

Immunocytochemistry/ Immunofluorescence: UbcH8/Ube2L6 Antibody (k1H3) - BSA Free [NBP1-04351]

Immunocytochemistry/Immunofluorescence: UbcH8/Ube2L6 Antibody (k1H3) [NBP1-04351] - ICC/IF analysis of UBE2L6 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human UBE2L6 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Immunohistochemistry-Paraffin: UbcH8/Ube2L6 Antibody (k1H3) - BSA Free [NBP1-04351]

Immunohistochemistry-Paraffin: UbcH8/Ube2L6 Antibody (k1H3) - BSA Free [NBP1-04351]

Immunohistochemistry-Paraffin: UbcH8/Ube2L6 Antibody (k1H3) [NBP1-04351] - Ube2L6 Antibody (k1H3) [NBP1-04351]-Paraffin embedded sections of human breast lobule tissue were incubated with anti-human UBE2L6 for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
Western Blot: UbcH8/Ube2L6 Antibody (k1H3)BSA Free [NBP1-04351]

Western Blot: UbcH8/Ube2L6 Antibody (k1H3)BSA Free [NBP1-04351]

Western Blot: UbcH8/Ube2L6 Antibody (k1H3) [NBP1-04351] - Ube2L6 Antibody (k1H3) [NBP1-04351]-Cell lysates of MCF7 (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human UBE2L6. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Western Blot: UbcH8/Ube2L6 Antibody (k1H3)BSA Free [NBP1-04351]

Western Blot: UbcH8/Ube2L6 Antibody (k1H3)BSA Free [NBP1-04351]

Western Blot: UbcH8/Ube2L6 Antibody (k1H3) [NBP1-04351] - The Cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human UBE2L6 antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: UBE2L6 Transfected 293T cell lysate

Applications for UbcH8/Ube2L6 Antibody (k1H3) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UbcH8

Ubiquitin-conjugating enzyme E2L6 (UBE2L6), also known as UbcH8, is a member of the E2 ubiquitin-conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination of a protein substrate requires the concerted action of 3 classes of enzymes: E1 ubiquitin-activating enzymes, E2 ubiquitin-conjugating enzymes, and E3 ubiquitin protein ligases. The E2 ubiquitin-conjugating enzyme is highly similar in primary structure to the enzyme encoded by UBE2L3 gene.

Alternate Names

RIG-B, UBE2L6

Gene Symbol

UBE2L6

UniProt

NP_004214 (Human)

Additional UbcH8 Products

Product Documents for UbcH8/Ube2L6 Antibody (k1H3) - BSA Free

Certificate of Analysis

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Product Specific Notices for UbcH8/Ube2L6 Antibody (k1H3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail