UBE2I/Ubc9 Antibody

Novus Biologicals | Catalog # NB300-812

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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human

Predicted:

Canine (100%), Mouse (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all UBE2I/Ubc9 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence SGIALSRLAQERK-C corresponding to N-Terminus according to NP_919235.1, NP_919236.1, NP_919237.1.

Specificity

This antibody is expected to recognize all reported isoforms (NP_003336.1, NP_919235.1, NP_919236.1 and NP_919237.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for UBE2I/Ubc9 Antibody

Western Blot: UBE2I/Ubc9 Antibody [NB300-812]

Western Blot: UBE2I/Ubc9 Antibody [NB300-812]

Western Blot: UBE2I/Ubc9 Antibody [NB300-812] - Staining of Rat Ovary lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry-Paraffin: UBE2I/Ubc9 Antibody [NB300-812]

Immunohistochemistry-Paraffin: UBE2I/Ubc9 Antibody [NB300-812]

Immunohistochemistry-Paraffin: UBE2I/Ubc9 Antibody [NB300-812] - Human breast cancer tissue. IHC-P image submitted by a verified customer review.
Western Blot: UBE2I/Ubc9 Antibody [NB300-812]

Western Blot: UBE2I/Ubc9 Antibody [NB300-812]

Western Blot: UBE2I/Ubc9 Antibody [NB300-812] - Staining of Human Kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: UBE2I/Ubc9 Antibody [NB300-812]

Immunohistochemistry-Paraffin: UBE2I/Ubc9 Antibody [NB300-812]

Immunohistochemistry-Paraffin: UBE2I/Ubc9 Antibody [NB300-812] - Staining of Human Kidney. Antibody at 0.3 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
UBE2I/Ubc9 Antibody

Western Blot: UBE2I/Ubc9 Antibody [NB300-812] -

Trim28 mediates the SUMOylation of alpha -Syn and tau.(A) Blocking SUMOylation – by either pharmacological inhibition using viomellein or siRNA-mediated suppression of the sole SUMO E2 ligase, UBC9 – decreases alpha -Syn and tau levels by western blot. (B) SUMO assay in human cells reveals that TRIM28 mediates the formation of SUMO2 adducts on alpha -Syn and tau. This effect is lost upon mutation of the RING domain of TRIM28 (TRIM28-Mut). (C) In vivo SUMO assay from denatured mouse brain lysates of WT and Trim28+/- mice. Snca-/- and Mapt-/- mice and IP: IgG serve as negative controls. *, **, *** and ns denote p<0.05, p<0.01, p<0.001 and p>0.05, respectively.Ablating endogenous Trim28 catalytic activity dramatically reduces its stability, concomitantly decreasing alpha -Syn and tau levels.(A), Structural rationale for targeting the RING domain of Trim28. Alignment of human TRIM28 (hsTRIM28) with mouse (mmTrim28) and Drosophila melanogaster (dmTRIM28) TRIM28 in addition to human TRIM32 (hsTRIM32). * denotes the sequence upon which modeling was conducted. Modeling of RING domain disruption in PyMOL using the TRIM32 RING domain (PDB: 5FEY). (B), Approach to mutate endogenous Trim28 catalytic activity and Sanger sequencing confirmation of mutation insertion. (C), Western blot and qPCR analysis of Trim28, Snca and Mapt transcripts in Trim28 E3 mutant heterozygous mice (Trim28E3MT/+) compared to littermate controls. In (C), n = 4–9 mice per genotype. Error bars denote s.e.m. **, **** and ns denote p<0.01, p<0.0001 and p>0.05, respectively. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29863470), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for UBE2I/Ubc9 Antibody

Application
Recommended Usage

Immunohistochemistry

1 ug/mL

Immunohistochemistry-Paraffin

1 ug/mL

Peptide ELISA

Detection limit 1:64000

Western Blot

0.1 - 0.3 ug/mL
Application Notes
WB: Approx. 17 kDa band observed in human kidney, lung and testis lysates and in mouse and rat heart, spleen and testis lysates (calculated MW of 18.0 kDa band according to human NP_003336.1). IHC-P: Human kidney show nuclear staining of some epithelial cells of glomeruli and renal tubules.

Reviewed Applications

Read 3 reviews rated 5 using NB300-812 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: UBE2I/Ubc9

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. Catalyzes the covalent attachment of ubiquitin-like protein SUMO-1 to other proteins. Forms a tight complex in association with two RAN GTPase interacting proteins, RANGAP1 and RANBP2. Interacts with E1A and E2A. Interacts with HIPK1 and HIPK2. Interacts with SIAH1 and PARP. Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.

Long Name

Ubiquitin-conjugating Enzyme E2I

Alternate Names

p18, SUMO-protein Ligase, Ubc9, UBCE9

Entrez Gene IDs

7329 (Human); 22196 (Mouse); 25573 (Rat)

Gene Symbol

UBE2I

UniProt

NP_003336. (Human)

Additional UBE2I/Ubc9 Products

Product Documents for UBE2I/Ubc9 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for UBE2I/Ubc9 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for UBE2I/Ubc9 Antibody

Customer Reviews for UBE2I/Ubc9 Antibody (3)

5 out of 5
3 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • UBE2I/Ubc9 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Breast cancer tissue
    Species: Human
    Verified Customer | Posted 05/03/2020
    UBE2I/Ubc9 Antibody NB300-812
  • UBE2I/Ubc9 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Hela whole cell lysate
    Species: Human
    Verified Customer | Posted 12/27/2019
    UBE2I/Ubc9 Antibody NB300-812
  • UBE2I/Ubc9 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: RCC-1 and SN12C
    Species: Human
    Verified Customer | Posted 10/28/2018
    UBE2I/Ubc9 Antibody NB300-812

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Protocols

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