UBR1 Antibody (2F5) - Azide and BSA Free

Novus Biologicals | Catalog # H00197131-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2F5

Format

Azide and BSA Free
View all UBR1 Primary Antibodies »

Product Specifications

Immunogen

UBR1 (NP_777576, 2 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ADEEAGGTERMEISAELPQTPQRLASWWDQQVDFYTAFLHHLAQLVPEIYFAEMDPDLEKQEESVQMSIFTPLEWYLFGEDPDICLEKLKHSGAFQLCG

Specificity

UBR1 - ubiquitin protein ligase E3 component n-recognin 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for UBR1 Antibody (2F5) - Azide and BSA Free

Western Blot: UBR1 Antibody (2F5) [H00197131-M01]

Western Blot: UBR1 Antibody (2F5) [H00197131-M01]

Western Blot: UBR1 Antibody (2F5) [H00197131-M01] - UBR1 monoclonal antibody (M01), clone 2F5 Analysis of UBR1 expression in Hela S3 NE.
Sandwich ELISA: UBR1 Antibody (2F5) [H00197131-M01] - Detection limit for recombinant GST tagged UBR1 is approximately 0.03ng/ml as a capture antibody.

Applications for UBR1 Antibody (2F5) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: UBR1

The N-end rule pathway is one proteolytic pathway of the ubiquitin system. The recognition component of this pathway, encoded by this gene, binds to a destabilizing N-terminal residue of a substrate protein and participates in the formation of a substrate-linked multiubiquitin chain. This leads to the eventual degradation of the substrate protein. The protein described in this record has a RING-type zinc finger and a UBR-type zinc finger. Mutations in this gene have been associated with Johanson-Blizzard syndrome.

Alternate Names

E3 ubiquitin-protein ligase UBR1, E3a ligase, EC 6.3.2.-, JBS, MGC142065, MGC142067, N-recognin-1, ubiquitin ligase E3 alpha-I, ubiquitin protein ligase E3 component n-recognin 1, ubiquitin-protein ligase E3-alpha, Ubiquitin-protein ligase E3-alpha-1, Ubiquitin-protein ligase E3-alpha-I

Entrez Gene IDs

197131 (Human)

Gene Symbol

UBR1

OMIM

243800 (Human)

UniProt

NP_777576 (Human)

Additional UBR1 Products

Product Documents for UBR1 Antibody (2F5) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for UBR1 Antibody (2F5) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for UBR1 Antibody (2F5) - Azide and BSA Free

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Protocols

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FAQs

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