UBR2 Antibody

Novus Biologicals | Catalog # NBP1-45243

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Cited:

Western Blot, Chemotaxis

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all UBR2 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence SCPKSGGDKSRAPT corresponding to internal region according to NP_056070.1.

Epitope

SCPKSGGDKSRAPT

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 28740232). Mouse reactivity reported in scientific literature (PMID: 27897407).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for UBR2 Antibody

Western Blot: UBR2 Antibody [NBP1-45243]

Western Blot: UBR2 Antibody [NBP1-45243]

UBR2-Antibody-Western-Blot-NBP1-45243-img0002.jpg
Immunohistochemistry-Paraffin: UBR2 Antibody [NBP1-45243]

Immunohistochemistry-Paraffin: UBR2 Antibody [NBP1-45243]

Immunohistochemistry-Paraffin: UBR2 Antibody [NBP1-45243] - Staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
UBR2-Antibody-NBP1-45243-img0003.jpg

Applications for UBR2 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

5ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.1 ug/ml
Application Notes
WB: Preliminary experiments gave bands at approx. 150 kDa band in rat testis lysates after 0.01 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 199 kDa band according to rat NP_001171542.1. The detected band was successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).

Reviewed Applications

Read 1 review rated 5 using NBP1-45243 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: UBR2

Proteolysis by the ubiquitin-proteasome system controls the concentration of many regulatory proteins. The selectivity of ubiquitylation is determined by ubiquitin E3 ligases, which recognize the substrate's destabilization signal, or degron. The E3 ligase UBR2 participates in the N-end rule pathway, which targets proteins bearing an N-terminal degron, or N-degron (Kwon et al., 2003 [PubMed 14585983]).[supplied by OMIM]

Alternate Names

bA49A4.1, C6orf133MGC71112, chromosome 6 open reading frame 133, dJ242G1.1, dJ392M17.3, DKFZp686C08114, E3 ubiquitin-protein ligase UBR2, EC 6.3.2.-, KIAA0349FLJ36357, N-recognin-2, RP3-392M17.3, ubiquitin protein ligase E3 component n-recognin 2, Ubiquitin-protein ligase E3-alpha-2, Ubiquitin-protein ligase E3-alpha-II

Entrez Gene IDs

23304 (Human); 224826 (Mouse)

Gene Symbol

UBR2

UniProt

NP_056070.1 (Human)

Additional UBR2 Products

Product Documents for UBR2 Antibody

Certificate of Analysis

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Product Specific Notices for UBR2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for UBR2 Antibody (1)

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  • UBR2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: 293 cells transfected with UBR2 and Mouse myoblasts (C2C12 cell lysate)
    Species: Mouse
    Verified Customer | Posted 09/06/2017
    1:1000 dilution in BSA, incubate overnight
    UBR2 Antibody NBP1-45243

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