UCH-L1/PGP9.5 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87334

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all UCH-L1/PGP9.5 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QEVSPKVYFMKQTIGNSCGTIGLIHAVANNQDKLGFEDGSVLKQFLSETEKMSPEDRAKCFEKNEAIQAAHDAVAQEGQCRVDDKVNFHFILFNNVDGHLYELDGRMPFPVNHGASSEDTLLKDAAKVCREFTEREQGEVRFSAVAL

Reactivity Notes

Reactivity reported in scientific literature (PMID: 20587347)

Marker

pan-Neuronal Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UCH-L1/PGP9.5 Antibody - BSA Free

Western Blot: UCH-L1/PGP9.5 Antibody [NBP1-87334]

Western Blot: UCH-L1/PGP9.5 Antibody [NBP1-87334]

Western Blot: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Analysis in human cell lines U-251MG and MCF-7. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody [NBP1-87334]

Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody [NBP1-87334]

Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Staining of human cell line U-251 MG shows localization to nucleoplasm and cytosol.
Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP1-87334]

Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP1-87334]

Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Staining in human cerebral cortex and liver tissues. Corresponding UCHL1 RNA-seq data are presented for the same tissues.
Western Blot: UCH-L1/PGP9.5 Antibody [NBP1-87334]

Western Blot: UCH-L1/PGP9.5 Antibody [NBP1-87334]

Western Blot: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Analysis in mouse cerebral cortex tissue.
Western Blot: UCH-L1/PGP9.5 Antibody [NBP1-87334]

Western Blot: UCH-L1/PGP9.5 Antibody [NBP1-87334]

Western Blot: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP1-87334]

Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP1-87334]

Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons and in neuropil.
Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP1-87334]

Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP1-87334]

Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP1-87334]

Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP1-87334]

Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Staining of human liver shows no positivity in hepatocytes, as expected.
Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP1-87334]

Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP1-87334]

Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Staining of human pancreas shows strong cytoplasmic positivity in islets of Langerhans.
Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP1-87334]

Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP1-87334]

Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Staining of human rectum shows strong cytoplasmic positivity in peripheral ganglion and peripheral nerves.

Applications for UCH-L1/PGP9.5 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UCH-L1/PGP9.5

Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1) is a soluble cytoplasmic protein found in neurons and in cells of the diffuse neuroendocrine system, and their respective tumors. Therefore, UCH-L1 has been widely used as a peripheral nerve fiber marker. UCHL-1 functions as a tissue-specific ubiquitin carboxyl terminal hydrolase isoenzyme involved in the processing of both ubiquitin precursors and ubiquitinated proteins.

UCH L1 is down-regulated in brains from Parkinson disease and Alzheimer disease patients, and certian site specific mutations in the UCHL1 gene can either increase or decrese the risk of Parkinson's and/or Alzheimer's neurodegenerative diseases.

Human UCHL 1 and the closely related UCHL3 protein have one of the most complicated knot structures ever discovered, with five knot crossings. This knot structure is expected to help the protein resist degradation in the proteasome.

Long Name

Ubiquitin C-terminal Hydrolase L1

Alternate Names

PARK5, PGP9.5, UCHL1

Gene Symbol

UCHL1

Additional UCH-L1/PGP9.5 Products

Product Documents for UCH-L1/PGP9.5 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for UCH-L1/PGP9.5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for UCH-L1/PGP9.5 Antibody - BSA Free

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Protocols

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