UCP2 Antibody

Novus Biologicals | Catalog # NB100-59742

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Predicted:

Canine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all UCP2 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DSVKQFYTKGSEH corresponding to internal region according to NP_003346.2.

Reactivity Notes

Predicted cross-reactivity based on sequence similarity: Canine.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UCP2 Antibody

Western Blot: UCP2 Antibody [NB100-59742]

Western Blot: UCP2 Antibody [NB100-59742]

Western Blot: UCP2 Antibody [NB100-59742] - Staining of rat adipose (A) and mouse spleen (B) lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence. Approx 37 kDa band observed in rat adipose and 28-30 kDa in mouse spleen lysates (calculated MW of 33.4kDa according to Mouse NP_035801.3 and 33.4kDa according to Rat NP_062227.2).
Immunocytochemistry/ Immunofluorescence: UCP2 Antibody [NB100-59742]

Immunocytochemistry/ Immunofluorescence: UCP2 Antibody [NB100-59742]

Immunocytochemistry/Immunofluorescence: UCP2 Antibody [NB100-59742] - Analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Applications for UCP2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry

6-7 ug/ml

Peptide ELISA

Detection limit 1:128000

Western Blot

0.3 - 1 ug/mL
Application Notes
WB: Approx 37kDa band observed in Rat Adipose and 28-30kDa in Mouse Spleen lysates (calculated MW of 33.4kDa according to Mouse NP_035801.3 and 33.4kDa according to Rat NP_062227.2).

Reviewed Applications

Read 2 reviews rated 3 using NB100-59742 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: UCP2

Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed in many tissues, with the greatest expression in skeletal muscle. It is thought to play a role in nonshivering thermogenesis, obesity and diabetes. Chromosomal order is 5'-UCP3-UCP2-3'.

Long Name

Uncoupling Protein 2

Alternate Names

SLC25A8, UCPH

Entrez Gene IDs

7351 (Human); 22228 (Mouse); 54315 (Rat)

Gene Symbol

UCP2

UniProt

P55851

Additional UCP2 Products

Product Documents for UCP2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for UCP2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Bcl-2 Proteins and Regulators

Customer Reviews for UCP2 Antibody (2)

3 out of 5
2 Customer Ratings
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Customer Images

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  • UCP2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Human primary fibroblast
    Species: Human
    Verified Customer | Posted 10/24/2020
    UCP2 in human primary fibroblasts PFA fixed. Donkey anti-Goat Alexa Fluor 647 detection.
    Fixation: PFA 4% 15 RT. Blocking/permeabilization: 1h in 10% DS in PBST (0.1% Triton). UCP2 antibody incubation O/N 4ºC 1/100 dilution in 10% DS in PBST (0.1%Triton). Secondary antibody: Donkey anti-Goat Alexa Fluor 647 1:500 1h RT.
    UCP2 Antibody NB100-59742
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to that that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Liver mitochondrial protein preparations
    Species: Rat
    Verified Customer | Posted 12/19/2014

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Protocols

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FAQs for UCP2 Antibody

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  • Q: Hi, I'd like to measure UCP2 levels in various mouse tissues by western blot. I was wondering which of your antibodies would be best for this purpose. In the literature I have seen some papers where they have isolated mitochondria to be able to see UCP2 and others where they have not. We would like to use frozen tissue samples, so isolating mitochondria will not be an option. We would like to use liver, muscle, pancreas, kidney, brown fat, subcutaneous fat, and gonadal fat. I'd appreciate any advice you might have.

    A: Regarding your question on our UCP2 antibodies, we do have a product (catalog # NB100-78377), but our lab confirmed that it has only been validated in mouse liver and brain and there is no guarantee on other tissues.

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