UNC13B Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-93337

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all UNC13B Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human UNC13B (NP_006368.3). MSLLCVRVKRAKFQGSPDKFNTYVTLKVQNVKSTTVAVRGDQPSWEQDFMFEISRLDLGLSVEVWNKGLIWDTMVGTVWIALKTIRQSDEEGPGEWSTLEAETLMKDDEICGTRNPTPHKILLDTRFELPFDIPEEEARYWTYKWEQINALGADNEYSSQEESQRKPLPTAAAQCSFEDPDSAVDDRDSDYRSETSNSFPPPYHTASQPNASVHQFPVPVRSPQQLLLQGSSRDSCNDSMQSYDLDYPERRAISPTSSSRYGSSCNVSQGSSQLSELDQYHEQDDDHRETDSIHSCHSSHSLSRDGQAGFGEQEKPLEVTGQAEKEAACEPKEMKEDATTHPPPDLVLQK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

181 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit UNC13B Antibody - Azide and BSA Free (NBP2-93337) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for UNC13B Antibody - Azide and BSA Free

Western Blot: UNC13B AntibodyAzide and BSA Free [NBP2-93337]

Western Blot: UNC13B AntibodyAzide and BSA Free [NBP2-93337]

Western Blot: UNC13B Antibody [NBP2-93337] - Analysis of extracts of various cell lines, using UNC13B at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry-Paraffin: UNC13B Antibody - Azide and BSA Free [NBP2-93337]

Immunohistochemistry-Paraffin: UNC13B Antibody - Azide and BSA Free [NBP2-93337]

Immunohistochemistry-Paraffin: UNC13B Antibody [NBP2-93337] - Immunohistochemistry of paraffin-embedded Human liver using UNC13B Rabbit pAb (NBP2-93337) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
UNC13B Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: UNC13B Antibody - Azide and BSA Free [NBP2-93337] -

Immunocytochemistry/ Immunofluorescence: UNC13B Antibody - Azide and BSA Free [NBP2-93337] - Immunofluorescence analysis of L929 cells using UNC13B Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for UNC13B Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: UNC13B

The UNC13B gene is expressed in the kidney cortical epithelial cells and is upregulated by hyperglycemia. The encoded protein shares a high level of similarity to the rat homolog, and contains 3 C2 domains and a diacylglycerol-binding C1 domain. Hyperglycemia i

Long Name

UNC-13 Homolog B

Alternate Names

hmunc13, MUNC13B, Unc13h2

Gene Symbol

UNC13B

Additional UNC13B Products

Product Documents for UNC13B Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for UNC13B Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Citations for UNC13B Antibody - Azide and BSA Free

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Protocols

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