VAMP-2 Antibody (GT766) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-13634

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # GT766

Format

Azide and BSA Free
View all VAMP-2 Primary Antibodies »

Product Specifications

Immunogen

full length human VAMP-2 recombinant protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Description

Centrifuge briefly prior to opening.

Scientific Data Images for VAMP-2 Antibody (GT766) - Azide and BSA Free

Western Blot: VAMP-2 Antibody (GT766) [NBP3-13634]

Western Blot: VAMP-2 Antibody (GT766) [NBP3-13634]

Western Blot: VAMP-2 Antibody (GT766) [NBP3-13634] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with VAMP-2 antibody [GT766] (NBP3-13634) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: VAMP-2 Antibody (GT766) [NBP3-13634]

Immunocytochemistry/ Immunofluorescence: VAMP-2 Antibody (GT766) [NBP3-13634]

Immunocytochemistry/Immunofluorescence: VAMP-2 Antibody (GT766) [NBP3-13634] - VAMP-2 antibody [GT766] detects VAMP-2 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VAMP-2 stained by VAMP-2 antibody [GT766] (NBP3-13634) diluted at 1:500. Red: Tau, stained by Tau antibody diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: VAMP-2 Antibody (GT766) [NBP3-13634]

Immunohistochemistry-Paraffin: VAMP-2 Antibody (GT766) [NBP3-13634]

Immunohistochemistry-Paraffin: VAMP-2 Antibody (GT766) [NBP3-13634] - VAMP-2 antibody [GT766] detects VAMP-2 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. VAMP-2 stained by VAMP-2 antibody [GT766] (NBP3-13634) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: VAMP-2 Antibody (GT766) [NBP3-13634]

Immunohistochemistry-Paraffin: VAMP-2 Antibody (GT766) [NBP3-13634]

Immunohistochemistry-Paraffin: VAMP-2 Antibody (GT766) [NBP3-13634] - VAMP-2 antibody [GT766] detects VAMP-2 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. VAMP-2 stained by VAMP-2 antibody [GT766] (NBP3-13634) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for VAMP-2 Antibody (GT766) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 20% Glycerol

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VAMP-2

Vesicle-associated membrane protein (VAMP), also known as synaptobrevin, is an 18 kDa integral membrane protein localized to the cytoplasmic surface of synaptic vesicle (2). VAMP consists of a proline-rich amino-terminal region, a highly conserved hydrophilic domain, followed by a transmembrane anchor and a carboxylterminal tail (3). Two VAMP homologs, VAMP1 and VAMP2, are differentially expressed in the nervous system (3). VAMP1 expression is localized to neurons involved in modulating overlapping patterns, whereas VAMP2 is found in neurons ssociated with autonomic, sensory and integrative functions. In non-neuronal tissues, VAMP1 is restricted to pancreas and kidney tubular cells, whereas VAMP2 is predominantly expressed in Langerhans islets and glomerular cells (4). Evidences for VAMP

Long Name

Vesicle-Associated Membrane Protein 2

Alternate Names

SYB2, VAMP2

Gene Symbol

VAMP2

Additional VAMP-2 Products

Product Documents for VAMP-2 Antibody (GT766) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for VAMP-2 Antibody (GT766) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for VAMP-2 Antibody (GT766) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review VAMP-2 Antibody (GT766) - Azide and BSA Free and earn rewards!

Have you used VAMP-2 Antibody (GT766) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies