VAMP-2 Antibody

Novus Biologicals | Catalog # NB300-595

Novus Biologicals
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Key Product Details

Species Reactivity

Mouse, Rat, Bovine, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

Synthetic peptide corresponding to residues M(1) S A T A A T V P P A A P A G E G G(18) C of rat VAMP-2.

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 10370136).

Localization

Type IV membrane protein. Neuronal synaptic vesicles

Marker

Synaptic Vesicles Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VAMP-2 Antibody

Western Blot: VAMP-2 Antibody [NB300-595]

Western Blot: VAMP-2 Antibody [NB300-595]

Western Blot: VAMP-2 Antibody [NB300-595] - Analysis was performed on tissues (40 ug lysate) of Mouse Cerebellum (Lane 1), Mouse Brain (Lane 2) and Rat Brain (Lane 3).
Flow Cytometry: VAMP-2 Antibody [NB300-595]

Flow Cytometry: VAMP-2 Antibody [NB300-595]

Flow Cytometry: VAMP-2 Antibody [NB300-595] - Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature.
Western Blot: VAMP-2 Antibody [NB300-595]

Western Blot: VAMP-2 Antibody [NB300-595]

Western Blot: VAMP-2 Antibody [NB300-595] - Analysis of rat brain extract.

Applications for VAMP-2 Antibody

Application
Recommended Usage

Flow Cytometry

3 - 5 ug / 10^6 cells

Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

1:10 - 1:500

Immunoprecipitation

1:10 - 1:500

Western Blot

0.3 ug/ml
Application Notes
IHC usage was reported in scientific literature (See Windoffer R, et al. 1999). WB: Detects an approx. 19 kDa protein representing VAMP 2 from rat brain whole protein extract.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VAMP-2

The vesicle associated membrane proteins (VAMP) or synaptobrevins are calcium binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP 25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimer's disease, VAMP levels of all isoforms appear to be significantly lowered. It is suggested that VAMP 2 is a resident protein of the insulin-sensitive glucose transporter type 4 (GLUT4) compartment and that it is required for GLUT4 vesicle incorporation into the cell surface in response to insulin.

Long Name

Vesicle-Associated Membrane Protein 2

Alternate Names

SYB2, VAMP2

Entrez Gene IDs

22318 (Mouse); 24803 (Rat)

Gene Symbol

VAMP2

Additional VAMP-2 Products

Product Documents for VAMP-2 Antibody

Certificate of Analysis

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Product Specific Notices for VAMP-2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for VAMP-2 Antibody

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Protocols

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