VAMP-2 Antibody

Novus Biologicals | Catalog # NBP3-13179

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Predicted:

Bovine (100%), Human (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all VAMP-2 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse VAMP-2. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for VAMP-2 Antibody

Western Blot: VAMP-2 Antibody [NBP3-13179]

Western Blot: VAMP-2 Antibody [NBP3-13179]

Western Blot: VAMP-2 Antibody [NBP3-13179] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with VAMP-2 antibody (NBP3-13179) diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: VAMP-2 Antibody [NBP3-13179]

Immunocytochemistry/ Immunofluorescence: VAMP-2 Antibody [NBP3-13179]

Immunocytochemistry/Immunofluorescence: VAMP-2 Antibody [NBP3-13179] - VAMP-2 antibody detects VAMP-2 protein at synaptic vesicles by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: VAMP-2 protein stained by VAMP-2 antibody (NBP3-13179) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue:Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: VAMP-2 Antibody [NBP3-13179]

Immunohistochemistry-Paraffin: VAMP-2 Antibody [NBP3-13179]

Immunohistochemistry-Paraffin: VAMP-2 Antibody [NBP3-13179] - VAMP-2 antibody detects VAMP-2 protein expression by immunohistochemical analysis. Sample:Paraffin-Embedded adult mouse retina. Green: VAMP-2 protein stained by VAMP-2 antibody (NBP3-13179) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Frozen: VAMP-2 Antibody [NBP3-13179]

Immunohistochemistry-Frozen: VAMP-2 Antibody [NBP3-13179]

Immunohistochemistry-Frozen: VAMP-2 Antibody [NBP3-13179] - VAMP-2 antibody detects VAMP-2 protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: VAMP-2 protein stained by VAMP-2 antibody (NBP3-13179) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: VAMP-2 Antibody [NBP3-13179]

Immunohistochemistry-Paraffin: VAMP-2 Antibody [NBP3-13179]

Immunohistochemistry-Paraffin: VAMP-2 Antibody [NBP3-13179] - VAMP-2 antibody detects VAMP-2 protein at cell membrane in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. VAMP-2 antibody (NBP3-13179) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: VAMP-2 Antibody [NBP3-13179]

Immunohistochemistry-Paraffin: VAMP-2 Antibody [NBP3-13179]

Immunohistochemistry-Paraffin: VAMP-2 Antibody [NBP3-13179] - VAMP-2 antibody detects VAMP-2 protein at cell membrane in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. VAMP-2 antibody (NBP3-13179) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: VAMP-2 Antibody [NBP3-13179]

Immunohistochemistry-Paraffin: VAMP-2 Antibody [NBP3-13179]

Immunohistochemistry-Paraffin: VAMP-2 Antibody [NBP3-13179] - VAMP-2 antibody detects VAMP-2 protein at cell membrane in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. VAMP-2 antibody (NBP3-13179) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for VAMP-2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VAMP-2

Vesicle-associated membrane protein (VAMP), also known as synaptobrevin, is an 18 kDa integral membrane protein localized to the cytoplasmic surface of synaptic vesicle (2). VAMP consists of a proline-rich amino-terminal region, a highly conserved hydrophilic domain, followed by a transmembrane anchor and a carboxylterminal tail (3). Two VAMP homologs, VAMP1 and VAMP2, are differentially expressed in the nervous system (3). VAMP1 expression is localized to neurons involved in modulating overlapping patterns, whereas VAMP2 is found in neurons ssociated with autonomic, sensory and integrative functions. In non-neuronal tissues, VAMP1 is restricted to pancreas and kidney tubular cells, whereas VAMP2 is predominantly expressed in Langerhans islets and glomerular cells (4). Evidences for VAMP

Long Name

Vesicle-Associated Membrane Protein 2

Alternate Names

SYB2, VAMP2

Gene Symbol

VAMP2

Additional VAMP-2 Products

Product Documents for VAMP-2 Antibody

Certificate of Analysis

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Product Specific Notices for VAMP-2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for VAMP-2 Antibody

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Protocols

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FAQs

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