VAMP-7 Antibody (158.2)

Western Blot: VAMP-7 Antibody (158.2) [NBP1-07118] -

Anterograde, but not retrograde, pathway regulators are recruited together with LAT at the immune synapse. (A) Still images from live TIRFM imaging of Jurkat cells co-expressing LAT-mCherry and GMAP210-GFP, VAMP7-GFP or Rab6-GFP, seeded on coverslips coated with anti-CD3 epsilon +anti-CD28 Abs. Pearson correlation coefficient is shown (Mean +/- SD). Dashed square indicate the magnified regions. Arrow heads point out the appearance and displacement in the evanescent field of vesicles-containing simultaneous or individual LAT and GMAP210/VAMP7 or Rab6, respectively. Scale bars = 5 μm. Inset scale bars = 2 μm. (B) Immunoblot of signalosomes prepared from Jurkat cells activated for 5, 10 or 15 min with anti-CD3 epsilon +anti-CD28 coated magnetic beads. IgG corresponds to Jurkat cells incubated with irrelevant mAb coated magnetic beads for 15 min. Proteins attached to the beads were purified by magnetic sorting after freezing and thawing the cells. Presence of the different proteins in the corresponding cell lysates (with detergent) are shown in “input" lanes. Data and images represent two independent experiments in (A,B). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33572370), licensed under a CC-BY license. Not internally tested by Novus Biologicals.