VCAM-1/CD106 Antibody (MR106) - BSA Free

Name: VCAM-1/CD106 Antibody (MR106) - BSA Free
Brand: Novus Biologicals
SKU: NBP1-43368
Price: 159 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-43368

Submit a review

Citations (1)

Product Documents

100% Guarantee

Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # MR106

Format

BSA Free

View all VCAM-1/CD106 Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody was CD106.

Reactivity Notes

This antibody is reactive to Rat.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Scientific Data Images for VCAM-1/CD106 Antibody (MR106) - BSA Free

Western Blot: VCAM-1/CD106 Antibody (MR106)BSA Free [NBP1-43368]

VCAM-1-CD106-Antibody-MR106-Western-Blot-NBP1-43368-img0005.jpg

Flow Cytometry: VCAM-1/CD106 Antibody (MR106) - BSA Free [NBP1-43368]

Flow Cytometry: VCAM-1/CD106 Antibody (MR106) [NBP1-43368] - Analysis using the Biotin conjugate of NBP1-43368. Staining of rat bone marrow cells with 0.125 ug of Mouse IgG1 kappa Isotype Control Biotin (left) or 0.125 ug of Anti-Rat CD106 (VCAM-1) Biotin (right) followed by Streptavidin PE.

Flow Cytometry: VCAM-1/CD106 Antibody (MR106) - BSA Free [NBP1-43368]

Flow Cytometry: VCAM-1/CD106 Antibody (MR106) [NBP1-43368] - Staining of rat bone marrow cells with 0.25 ug of Mouse IgG1 kappa Isotype Control Purified (open histogram) or 0.25 ug of Anti-Rat CD106 (VCAM-1) Purified (filled histogram) followed by Anti-Mouse IgG Biotin and Streptavidin PE. Cells in the large scatt

Western Blot: VCAM-1/CD106 Antibody (MR106) - BSA Free [NBP1-43368] -

Western blot analyses of protein expressions of glutathione peroxidase-1 (GPx1), vascular cell adhesion molecule 1 (VCAM-1), inducible nitric oxide (iNOS), nuclear factor (NF-kappa B), matrix metalloproteinase-9 (MMP-9), and surfactant protein A and D (SP-A and SP-D) in lung (n = 3; * and # signify P < 0.05 and P < 0.001, respectively)

View 1 more images

Applications for VCAM-1/CD106 Antibody (MR106) - BSA Free

Application

Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000

Application Notes

This MR106 antibody has been tested by flow cytometric analysis of rat bone marrow cells. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

Build Your Panel Now

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: VCAM-1/CD106

The MR106 monoclonal antibody reacts with rat CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1), a 110 kDa transmembrane glycoprotein expressed by myeloid lineage and bone marrow stromal cells. Endothelial cells constitutively express low levels of CD106 and further upregulate VCAM-1 upon cytokine stimulation. CD106 binds to integrin alpha4beta1 (VLA-4, CD49d/CD29) and Integrin alpha4beta7 (LPAM-1) and these interactions in the bone marrow and thymus are important for early lymphocyte and myeloid development. Cytokine-mediated upregulation of CD106 on endothelial cells suggests a role for this antigen in the inflammatory response.

Long Name

Vascular Cell Adhesion Molecule 1

Alternate Names

CD106, VCAM1

Gene Symbol

VCAM1

Additional VCAM-1/CD106 Products

Product Documents for VCAM-1/CD106 Antibody (MR106) - BSA Free

Download Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for VCAM-1/CD106 Antibody (MR106) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.