VDP p115 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-38401

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all VDP p115 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: AKSVEVQGETETIIATKTTDVEGRLSALLQETKELKNEIKALSEERTAIKEQLDSSNSTIAILQTEKDKLELEITDSKKEQDDLLVLL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VDP p115 Antibody - BSA Free

Western Blot: VDP p115 Antibody [NBP2-38401]

Western Blot: VDP p115 Antibody [NBP2-38401]

Western Blot: VDP p115 Antibody [NBP2-38401] - Analysis using Anti-USO1 antibody NBP2-38401 (A) shows similar pattern to independent antibody NBP2-55590 (B).
Immunocytochemistry/ Immunofluorescence: VDP p115 Antibody [NBP2-38401]

Immunocytochemistry/ Immunofluorescence: VDP p115 Antibody [NBP2-38401]

Immunocytochemistry/Immunofluorescence: VDP p115 Antibody [NBP2-38401] - Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.
Immunohistochemistry-Paraffin: VDP p115 Antibody [NBP2-38401]

Immunohistochemistry-Paraffin: VDP p115 Antibody [NBP2-38401]

Immunohistochemistry-Paraffin: VDP p115 Antibody [NBP2-38401] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.

Applications for VDP p115 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VDP p115

p115 (Vesicle docking protein p115) is a peripheral membrane protein that is located on the Golgi complex. p115 exists as a homodimer with two globular heads, an extended coiled-coil tail, followed by an acidic domain at the extreme C terminus. p115 is homologous to a yeast protein, Uso1p, which is required for ER to Golgi transport. p115 likely plays an important role in vesicle transportation from the ER to the cis-Golgi comparments.

Alternate Names

p115, Transcytosis-associated protein, USO1 homolog, vesicle docking protein (yeast), USO1 vesicle docking protein homolog (yeast), vesicle docking protein, vesicle docking protein p115

Gene Symbol

USO1

UniProt

O60763 (Human)

Additional VDP p115 Products

Product Documents for VDP p115 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for VDP p115 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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