VEGFR1/Flt-1 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-527

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat

Predicted:

Chicken (94%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Flow Cytometry, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all VEGFR1/Flt-1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal region of the mouse VEGF Receptor 1 protein (between residues 800-900) [Uniprot: P35969]. There is 87% identity between the immunogen used for this production and the VEGF Receptor 2 protein.

Localization

Type 1 membrane protein (isoform Flt1)

Marker

Endothelial Cell Marker

Specificity

This antibody targets VEGFR-1 but has significant cross-reactivity with the VEGFR-2 protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VEGFR1/Flt-1 Antibody - BSA Free

Western Blot: VEGFR1/Flt-1 Antibody [NB100-527]

Western Blot: VEGFR1/Flt-1 Antibody [NB100-527]

Western Blot: VEGF R1/Flt-1 Antibody [NB100-527] - Chimeric CSF-1R/VEGFR-2 detection in transfected lysates.
Immunocytochemistry/ Immunofluorescence: VEGFR1/Flt-1 Antibody [NB100-527]

Immunocytochemistry/ Immunofluorescence: VEGFR1/Flt-1 Antibody [NB100-527]

Immunocytochemistry/Immunofluorescence: VEGFR1/Flt-1 Antibody [NB100-527] - VEGF R1/Flt-1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody [NB100-527]

Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody [NB100-527]

Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody [NB100-527] - Analysis of FFPE human breast carcinoma tissue section using 1:500 dilution of VEGFR1/Flt-1 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This VEGFR1/Flt-1 antibody generated an expected membrane cytoplasmic staining of VEGFR1 protein in the cancer cells (punctate appearance typical of receptors). The tumor stroma/stromal cells did not show VEGFR1/Flt-1immunopositivity.
Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody [NB100-527]

Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody [NB100-527]

Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody [NB100-527] - Analysis of a FFPE human breast carcinoma tissue section using 1:500 dilution of VEGFR1/Flt-1 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This VEGFR1/Flt-1 antibody generated an expected membrane cytoplasmic staining of VEGFR1 protein in the cancer cells. The tumor stroma/stromal cells did not show VEGFR1/Flt-1immunopositivity. Staining was performed by Histowiz.
VEGFR1/Flt-1 Antibody - BSA Free Western Blot: Rabbit Polyclonal VEGFR1/Flt-1 Antibody [NB100-527]

Western Blot: Rabbit Polyclonal VEGFR1/Flt-1 Antibody [NB100-527]

VEGFR1 overexpression in K562 cells. Image from a verified customer review.

Applications for VEGFR1/Flt-1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500

Immunohistochemistry

1:250 - 1:500

Immunohistochemistry-Paraffin

1:250 - 1:500

Western Blot

1:100 - 1:2000

Reviewed Applications

Read 1 review rated 5 using NB100-527 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: VEGFR1/Flt-1

VEGF Receptor 1 (vascular endothelial growth factor receptor 1 or VEGFR1; also called Flt1 in mouse) is a tyrosine-protein kinase which acts as a cell-surface receptor for VEGFA, VEGFB and PGF ligands for playing an essential role in the embryonic vasculature development and regulation of angiogenesis (normal as well as cancerous tissues), cell survival, migration, macrophage function, chemotaxis, and tumor invasion. VEGFR1 is a 180-185 kD glycoprotein expressed primarily in vascular endothelial cells and also found in a wide range of non-endothelial cells, such as monocytes and macrophages, human trophoblasts, renal mesangial cells, vascular smooth muscle cells, dendritic cells and different human tumour cell types. Interestingly, VEGFR1 acts as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells, whereas it promotes endothelial cell proliferation, survival and angiogenesis in adults. VEGFR1 expression is regulated by hypoxia through HREs in VEGFR1 promoter. It exists in an inactive conformation in the absence of bound ligand and binding of VEGFA, VEGFB or PGF leads to its dimerization followed by tyrosine residue autophosphorylation mediated activation. VEGFR1 phosphorylation sites includes Tyr794, Tyr1169, Tyr1213, Tyr1242, Tyr1327, Tyr1333 etc. and VEGFR1 phosphorylation pattern depends on the ligand, e.g. PlGF, but not VEGFA, induces phosphorylation of Tyr1309. VEGFR1 has been shown to exert its diverse biological activities through its effects on multiple signal transduction pathways including VEGF, KDR, PLCG, PI3K, MAPK etc.

Long Name

Vascular Endothelial Growth Factor Receptor 1

Alternate Names

Flt-1, FLT1, FRT, VEGF R1, VEGFR-1

Gene Symbol

FLT1

Additional VEGFR1/Flt-1 Products

Product Documents for VEGFR1/Flt-1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for VEGFR1/Flt-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for VEGFR1/Flt-1 Antibody - BSA Free

Customer Reviews for VEGFR1/Flt-1 Antibody - BSA Free (1)

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Customer Images

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  • VEGFR1/Flt-1 Antibody - BSA Free
    Name: Anonymous
    Application: Western Blot
    Sample Tested: k562 cells
    Species: Human
    Verified Customer | Posted 06/19/2025
    VEGFR1 overexpression in K562 cells
    VEGFR1/Flt-1 Antibody - BSA Free NB100-527

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Protocols

View specific protocols for VEGFR1/Flt-1 Antibody - BSA Free (NB100-527):

Immunocytochemistry Protocol

Culture cells to appropriate density in 35 mm culture dishes or 6-well plates.

1. Remove culture medium and wash the cells briefly in PBS. Add 10% formalin to the dish and fix at room temperature for 10 minutes.
2. Remove the formalin and wash the cells in PBS.
3. Permeablize the cells with 0.1% Triton X100 or other suitable detergent for 10 min.
4. Remove the permeablization buffer and wash three times for 10 minutes each in PBS. Be sure to not let the specimen dry out.
5. To block nonspecific antibody binding, incubate in 10% normal goat serum from 1 hour to overnight at room temperature.
6. Add primary antibody at appropriate dilution and incubate overnight at 4C.
7. Remove primary antibody and replace with PBS. Wash three times for 10 minutes each.
8. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
9. Remove secondary antibody and replace with PBS. Wash three times for 10 minutes each.
10. Counter stain DNA with DAPi if required.

Immunohistochemistry-Paraffin Embedded Sections

Antigen Unmasking:
Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes (keep slides in the sodium citrate buffer at all times).

Staining:
1. Wash sections in deionized water three times for 5 minutes each.
2. Wash sections in PBS for 5 minutes.
3. Block each section with 100-400 ul blocking solution (1% BSA in PBS) for 1 hour at room temperature.
4. Remove blocking solution and add 100-400 ul diluted primary antibody. Incubate overnight at 4 C.
5. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
6. Add 100-400 ul HRP polymer conjugated secondary antibody. Incubate 30 minutes at room temperature.
7. Wash sections three times in wash buffer for 5 minutes each.
8. Add 100-400 ul DAB substrate to each section and monitor staining closely.
9. As soon as the sections develop, immerse slides in deionized water.
10. Counterstain sections in hematoxylin.
11. Wash sections in deionized water two times for 5 minutes each.
12. Dehydrate sections.
13. Mount coverslips.


Western Blot Protocol

1. Perform SDS-PAGE on samples to be analyzed, loading 10-25 ug of total protein per lane.
2. Transfer proteins to PVDF membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
3. Stain the membrane with Ponceau S (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
4. Rinse the blot TBS -0.05% Tween 20 (TBST).
5. Block the membrane in 5% Non-fat milk in TBST (blocking buffer) for at least 1 hour.
6. Wash the membrane in TBST three times for 10 minutes each.
7. Dilute primary antibody in blocking buffer and incubate overnight at 4C with gentle rocking.
8. Wash the membrane in TBST three times for 10 minutes each.
9. Incubate the membrane in diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) for 1 hour at room temperature.
10. Wash the blot in TBST three times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturer's instructions.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for VEGFR1/Flt-1 Antibody - BSA Free

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  • Q: I am studying the regulation of PLCg during fertilization in the starfish. I am interested in trying your anti-FLT1 polyclonal antibody. Your information sheet says that the immunogen for this antibody is "A synthetic peptide corresponding to a sequence in the middle region of human FLT1". Since I will be using this antibody in starfish eggs, I compared the starfish protein sequence to the human protein sequence and found areas of high identity between the two and I want to use an antibody that is expected to bind to amino acids common to both. Therefore, I would like some more information to match the immunogen to the starfish protein. Can you tell me specifically which amino acids of human FLT1 were used to make the antibody?

    A: The immunogen sequence to this product is proprietary. I can tell you it falls between amino acids 800-900 of the mouse FLT1 protein [UniProt# P35969].

  • Q: Would you be able to tell me the concentration of the current lot of NB100-527 - VEGFR1/VEGFR2 Ab?

    A:

    The concentration of NB100-527 is 1mg/mL

  • Q: I am studying the regulation of PLCg during fertilization in the starfish. I am interested in trying your anti-FLT1 polyclonal antibody. Your information sheet says that the immunogen for this antibody is "A synthetic peptide corresponding to a sequence in the middle region of human FLT1". Since I will be using this antibody in starfish eggs, I compared the starfish protein sequence to the human protein sequence and found areas of high identity between the two and I want to use an antibody that is expected to bind to amino acids common to both. Therefore, I would like some more information to match the immunogen to the starfish protein. Can you tell me specifically which amino acids of human FLT1 were used to make the antibody?

    A: The immunogen sequence to this product is proprietary. I can tell you it falls between amino acids 800-900 of the mouse FLT1 protein [UniProt# P35969].

  • Q: Would you be able to tell me the concentration of the current lot of NB100-527 - VEGFR1/VEGFR2 Ab?

    A:

    The concentration of NB100-527 is 1mg/mL

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