VEGFR3/Flt-4 Antibody (9D9) [Biotin]

Novus Biologicals | Catalog # NBP1-18652

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Biotin

Antibody Source

Monoclonal Mouse IgG1 Clone # 9D9
View all VEGFR3/Flt-4 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human soluble VEGFR-3/FLT-4

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Clone #1 changed to 9D9 as a unique identifier.

Applications for VEGFR3/Flt-4 Antibody (9D9) [Biotin]

Application
Recommended Usage

ELISA

1-10 ug/ml

Immunoprecipitation

1-5 ug/ml

Western Blot

2-5 ug/ml
Application Notes
ELISA: To detect human VEGFR-3/FLT-4 by direct ELISA a concentration of 0.5 - 1.5 ug/ml can be used. This purified IgG, in combination with compatible secondary reagents, allows the detection of 0.25- 0.5 ng/well rhVEGFR-3/FLT-4. For Western blot analysis, the antibody can be used at 0.5 - 1 ug/ml with the appropriate secondary reagents to detect human VEGFR-3/FLT-4. Depending on the visualisation method the detection limit for rh VEGFR-3/FLT-4 is approximately 5 ng/lane under reducing conditions.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

Formulation

PBS and 50X BSA

Preservative

0.02% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VEGFR3/Flt-4

Vascular endothelial growth factors (VEGFs) are a family of closely related growth factors having a conserved pattern of eight cysteine esidues and sharing common VEGF receptors. VEGFs stimulate the proliferation of endothelial cells, induce angiogenesis, and increase vascular permeability in both large and small vessels. The mitogenic activity of VEGFs appears to be mediated by specific VEGF receptors. VEGF Receptor 3 is one of the five receptor tyrosine kinases (RTKs) (VEGF Receptor 1/Flt1, VEGF Receptor 2/KDR/Flk1, VEGF Receptor 3/Flt4, tie1 and tek/tie2) whose expression is almost exclusively restricted to endothelial cells.

Long Name

Vascular Endothelial Growth Factor Receptor 3

Alternate Names

Flt-4, FLT4, VEGF R3

Gene Symbol

FLT4

Additional VEGFR3/Flt-4 Products

Product Documents for VEGFR3/Flt-4 Antibody (9D9) [Biotin]

Certificate of Analysis

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Product Specific Notices for VEGFR3/Flt-4 Antibody (9D9) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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