Versican Antibody (6D8K9)

Novus Biologicals | Catalog # NBP3-15841

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6D8K9 expressed in HEK293
View all Versican Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 3200-3396 of human Versican (P13611). QGAHLTSILSHEEQMFVNRVGHDYQWIGLNDKMFEHDFRWTDGSTLQYENWRPNQPDSFFSAGEDCVVIIWHENGQWNDVPCNYHLTYTCKKGTVACGQPPVVENAKTFGKMKPRYEINSLIRYHCKDGFIQRHLPTIRCLGNGRWAIPKITCMNPSAYQRTYSMKYFKNSSSAKDNSINTSKHDHRWSRRWQESRR

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

373 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Versican Antibody (6D8K9)

Western Blot: Versican Antibody (6D8K9) [NBP3-15841]

Western Blot: Versican Antibody (6D8K9) [NBP3-15841]

Western Blot: Versican Antibody (6D8K9) [NBP3-15841] - Analysis of extracts of various cell lines, using Versican antibody (NBP3-15841) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
Versican Antibody (6D8K9)

Immunocytochemistry/ Immunofluorescence: Versican Antibody (6D8K9) [NBP3-15841] -

Immunocytochemistry/ Immunofluorescence: Versican Antibody (6D8K9) [NBP3-15841] - Immunofluorescence analysis of NIH-3T3 cells using Versican Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: Versican Antibody (6D8K9) [NBP3-15841]

Immunohistochemistry-Paraffin: Versican Antibody (6D8K9) [NBP3-15841]

Immunohistochemistry-Paraffin: Versican Antibody (6D8K9) [NBP3-15841] - Mouse brain using Versican Rabbit mAb (NBP3-15841) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: Versican Antibody (6D8K9) [NBP3-15841]

Immunohistochemistry-Paraffin: Versican Antibody (6D8K9) [NBP3-15841]

Immunohistochemistry-Paraffin: Versican Antibody (6D8K9) [NBP3-15841] - Rat kidney using Versican Rabbit mAb (NBP3-15841) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: Versican Antibody (6D8K9) [NBP3-15841]

Immunohistochemistry-Paraffin: Versican Antibody (6D8K9) [NBP3-15841]

Immunohistochemistry-Paraffin: Versican Antibody (6D8K9) [NBP3-15841] - Human esophageal cancer using Versican Rabbit mAb (NBP3-15841) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for Versican Antibody (6D8K9)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunocytochemistry/ Immunofluorescence

1:50 -1:200

Immunohistochemistry

1:200 - 1:2000

Immunohistochemistry-Paraffin

1:200 - 1:2000

Western Blot

1:1000 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Versican

Versican is a member of the aggrecan/versican proteoglycan family. The protein encoded is a large chondroitin sulfate proteoglycan and is a major component of the extracellular matrix. This protein is involved in cell adhesion, proliferation, proliferation, migration and angiogenesis and plays a central role in tissue morphogenesis and maintenance. Mutations in this gene are the cause of Wagner syndrome type 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

CSPG2, GHAP, PG-M, VCAN

Gene Symbol

VCAN

Additional Versican Products

Product Documents for Versican Antibody (6D8K9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Versican Antibody (6D8K9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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