Vinculin Antibody (hVIN-1)

Novus Biologicals | Catalog # NB600-1293

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Amphibian, Bovine, Canine, Chicken, Turkey

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Single Cell Western

Cited:

Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # hVIN-1
View all Vinculin Primary Antibodies »

Product Specifications

Immunogen

Purified human vinculin from uterus.

Reactivity Notes

Frog (100%). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Localization

Cytoplasm; cytoskeleton. Cell junction; adherens junction; cytoplasmic side. Cytoplasmic face of adhesion plaques.

Marker

Focal Adhesion Marker

Specificity

Specifically labels vinculin at cell-cell and cell-substrate contacts. Shows cross-reactivity with smooth muscle metavinculin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

116 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Vinculin Antibody (hVIN-1)

Western Blot: Vinculin Antibody (hVIN-1) [NB600-1293]

Western Blot: Vinculin Antibody (hVIN-1) [NB600-1293]

Western Blot: Vinculin Antibody (hVIN-1) [NB600-1293] - Cell line lysates were separated on SDS-PAGE and probed with 1:200 Monoclonal Anti-Vinculin Clone: hVIN-1. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.Lanes:1.HeLa2.COS73.NIH-3T34.RAT25.CHO6.MDBK7.MDCK
Immunocytochemistry/ Immunofluorescence: Vinculin Antibody (hVIN-1) [NB600-1293]

Immunocytochemistry/ Immunofluorescence: Vinculin Antibody (hVIN-1) [NB600-1293]

Vinculin-Antibody-hVIN-1-Immunocytochemistry-Immunofluorescence-NB600-1293-img0004.jpg
Immunohistochemistry: Vinculin Antibody (hVIN-1) [NB600-1293]

Immunohistochemistry: Vinculin Antibody (hVIN-1) [NB600-1293]

Immunohistochemistry: Vinculin Antibody (hVIN-1) [NB600-1293] - Enhanced Validation-By Independent Antibodies:Immunohistochemistry. Formalin-fixed, paraffin-embedded Rat Heart sections stained with 15 ug/mL Anti-Vinculin antibody produced in Rabbit (Cat. No. V4139) (A). The antibody was developed using Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat (Cat. No. F9887), and 15 ug/mL Monoclonal Anti-Vinculin antibody produced in Mouse, Clone: hVIN1 (Cat. No. V9131) (B). The antibody was developed using Rabbit Anti-Mouse IgG-Cy3 conjugate antibody.Results:Two Anti-Vinculin antibodies, V4139 (A) and V9131 (B), target different regions of Vinculin show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Vinculin Antibody (hVIN-1)

Western Blot: Vinculin Antibody (hVIN-1) [NB600-1293] -

A1B decreases TLR4 protein content in microglia chronically treated with LPS. (A) N9 cells seeded at 1 × 105 cells per well on poly-d-lysine-coated glass cover slides in 6-well plates were pre-treated for 4 h with DMSO or with 0.5 uM K-604, then exposed with or without 200 ng/mL LPS for 24 h. Double immunofluorescence staining for TLR4 and for the plasma membrane marker N-cadherin was then performed. (i) Timeline of the experiment. (ii) Representative images demonstrating TLR4 distribution in N9 cells. (iii) Quantification of total TLR4 relative fluorescence intensity per cell, n = 15 cells/treatment group. (iv) Quantification of IM/PM TLR4 fluorescence intensity ratio. A total of 15 cells per group were analyzed. The TLR4 signals overlapping with that of N-cadherin are considered as TLR4 at the PM, while those not overlapping are considered as TLR4 at the IM. (B) N9 microglial cells were seeded at 2 × 105 cells per well onto 6-well plates in RPMI-1640 with 10% serum. N9 cells were treated with DMSO (control group) or 0.5 uM K-604 for 4 h, then treated with or without 200 ng/mL LPS for 24 and 48 h. At different time points, cells were harvested for protein isolation and TLR4 Western blot analyses. n = 3 replicates. Vinculin was used as the protein loading control. (i) Timeline of experiment. (ii) Western blot. (iii) Quantitation of Western blot. * p < 0.05; ** p < 0.01; **** p < 0.0001. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/24/6/5616), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Vinculin Antibody (hVIN-1)

Immunocytochemistry/ Immunofluorescence: Vinculin Antibody (hVIN-1) [NB600-1293] -

Reduction of directionality and focal adhesion turnover by low eribulin concentrations(A) Effect of eribulin on MTOC directionality during wound healing. Percentage of cells with MTOC facing the wound (bottom). Values are mean +/- SEM (n = 3) **P < 0.01. (B) immunofluorescence images of LM8 cells treated with 0 nM, 1 nM, or 10 nM eribulin and stained for vinculin (red) and nucleus (blue) (left). Dotted line shows the cell shape. Scale bar: 10 μm. Quantitative analysis of the area of vinculin staining (right). Values are mean +/- SEM (≥30 cells per group). **P < 0.01. (C) Immunofluorescence images of LM8 cells treated with 0 nM, 1 nM, or 10 nM eribulin and stained for Tyr397-phosphorylated FAK (green), actin (red), and nucleus (blue) (left). Dotted line shows the cell shape. Scale bar: 10 μm. Quantitative analysis of the area of Tyr397-phosphorylated FAK staining (right). Values are mean +/- SEM (≥30 cells per group). *P < 0.05. Eribulin treatment shrank the Tyr397-phosphorylated FAK staining area in a dose-dependent manner. (D) Western blot of Tyr397-phosphorylated FAK in LM8 cells treated with eribulin (top). Quantitative densitometric analysis of the ratio of Tyr397-phosphorylated FAK to total FAK (bottom). Values are mean +/- SEM (n = 3). *P < 0.05. (E) Immunofluorescence images of LM8 cells treated with 0 nM or 10 nM eribulin and stained for APC (red), alpha -tubulin (green), and nucleus (blue). Scale bar: 10 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30719211), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Vinculin Antibody (hVIN-1)

Application
Recommended Usage

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

1:10 - 1:500

Single Cell Western

1:30

Western Blot

1:200 - 1:400
Application Notes
See Simple Western Antibody Database for Simple Western validation: tested in HeLa lysate; separated by size; antibody dilution of 1:5; matrix was 12-230 kDa. Single Cell Western reported by an internal validation on treated LNCap cells at a 1:30 dilution

Reviewed Applications

Read 1 review rated 5 using NB600-1293 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.09% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Vinculin

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. (provided by RefSeq)

Long Name

Metavinculin

Alternate Names

MVCL, VCL

Entrez Gene IDs

7414 (Human); 22330 (Mouse); 305679 (Rat)

Gene Symbol

VCL

UniProt

P18206

Additional Vinculin Products

Product Documents for Vinculin Antibody (hVIN-1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Vinculin Antibody (hVIN-1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Vinculin Antibody (hVIN-1)

Customer Reviews for Vinculin Antibody (hVIN-1) (1)

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Customer Images

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  • Vinculin Antibody (hVIN-1)
    Name: Qi Miao
    Application: Immunocytochemistry
    Sample Tested: skin keratinocytes
    Species: Mouse
    Verified Customer | Posted 04/26/2017
    Mouse primary keratinocytes stained with the clone hVIN-1
    Vinculin Antibody (hVIN-1) NB600-1293

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Vinculin Antibody (hVIN-1)

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  • Q: Do you have any 100 coda or bigger controls?

    A:

    I can recommend a couple of high weight antibodies that are conserved in most samples. Please verify the presence of the protein before using as a loading control. PARP1 or PARP is approximately 113kDa, and you may view the products we offer to PARP using this link. Vinculin is approximately 116kDa, and you may view the products we offer to Vinculin using this link.

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