VIP Antibody (2) - BSA Free
Novus Biologicals | Catalog # NBP1-05163
![Immunocytochemistry/ Immunofluorescence: VIP Antibody (2) - BSA Free [NBP1-05163]](https://beta-resources.rndsystems.com/images/products/VIP-Antibody-2-Immunocytochemistry-Immunofluorescence-NBP1-05163-img0005.jpg "Immunocytochemistry/ Immunofluorescence: VIP Antibody (2) - BSA Free [NBP1-05163]")
Key Product Details
Species Reactivity
Validated:
Human, Rat, Porcine, Bovine
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 2
Format
BSA Free
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Product Specifications
Immunogen
Vasoactive intestinal peptide
Specificity
Binds to human, bovine, porcine or rat VIP, but does not bind to PHM or PACAP
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Novus Biologicals Mouse VIP Antibody (2) - BSA Free (NBP1-05163) is a monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-VIP Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for VIP Antibody (2) - BSA Free
Immunocytochemistry/ Immunofluorescence: VIP Antibody (2) - BSA Free [NBP1-05163]
Immunocytochemistry/Immunofluorescence: VIP Antibody (2) [NBP1-05163] - Staining in human pancreas tissue. Signal is strong in nerves of exocrine part of pancreas. Dilution: 1:100.![Immunohistochemistry-Paraffin: VIP Antibody (2) - BSA Free [NBP1-05163]](https://beta-resources.rndsystems.com/images/products/VIP-Antibody-2-BSA-Free-Immunohistochemistry-Paraffin-NBP1-05163-img0006.jpg "Immunohistochemistry-Paraffin: VIP Antibody (2) - BSA Free [NBP1-05163]")
Immunohistochemistry-Paraffin: VIP Antibody (2) - BSA Free [NBP1-05163]
Immunohistochemistry-Paraffin: VIP Antibody (2) - BSA Free [NBP1-05163] - Formalin fixed paraffin-embedded human pancreas tissue. Dilution: 1:100![Immunohistochemistry-Paraffin: VIP Antibody (2) - BSA Free [NBP1-05163]](https://beta-resources.rndsystems.com/images/products/VIP-Antibody-2-Immunohistochemistry-Paraffin-NBP1-05163-img0002.jpg "Immunohistochemistry-Paraffin: VIP Antibody (2) - BSA Free [NBP1-05163]")
Immunohistochemistry-Paraffin: VIP Antibody (2) - BSA Free [NBP1-05163]
Immunohistochemistry-Paraffin: VIP Antibody (2) [NBP1-05163] - Staining of human appendix tissue. Dilution: 1:100![Immunohistochemistry-Paraffin: VIP Antibody (2) - BSA Free [NBP1-05163]](https://beta-resources.rndsystems.com/images/products/VIP-Antibody-2-Immunohistochemistry-Paraffin-NBP1-05163-img0004.jpg "Immunohistochemistry-Paraffin: VIP Antibody (2) - BSA Free [NBP1-05163]")
Immunohistochemistry-Paraffin: VIP Antibody (2) - BSA Free [NBP1-05163]
Immunohistochemistry-Paraffin: VIP Antibody (2) [NBP1-05163] - Staining of human appendix tissue. Dilution: 1:100Applications for VIP Antibody (2) - BSA Free
Application
Recommended Usage
ELISA
1:1000
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:100
Immunohistochemistry-Paraffin
1:100
Western Blot
1:100-1:2000
Application Notes
ELISA: A dilution guideline of 1/1000 applies to VIP coated directly at 2 ug/mL. For glutaraldehyde-linked VIP-ovalbumin (molar ratio 4:1) coated at 2 ug/mL, the dilution is 1/32,000.
WB: NBP1-05163 can be used in Western blotting.
IHC: NBP1-05163 was used in IHC in formaline fixed paraffin embedded tissue with a dilution of 1:100.
IF: NBP1-05163 was used in IF with a dilution of 1:100.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM Phosphate (pH 7.4) and 0.5M NaCl
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: VIP
Long Name
Vasoactive Intestinal Peptide
Alternate Names
MGC13587
Gene Symbol
VIP
Additional VIP Products
Product Documents for VIP Antibody (2) - BSA Free
Product Specific Notices for VIP Antibody (2) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for VIP Antibody (2) - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for VIP Antibody (2) - BSA Free
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Q: I am interested in the NBP1-05163, your anti-VIP mAb. I noticed that the mAb has been tested in a variety of applications, but I'm curious if you are aware of it being used in mice, and more importantly in flow cytometry and IF?
A: In regards to your inquiry unfortunately this antibody has only been tested in ELISA and we have not tested it in Flow cytometry or IF. Due to this antibody being of a mouse host we have not tested on mouse samples due to high background potential. Unfortunately we do not have any samples we can provide, but we do have our Innovator's Reward if you wish to test this in untested species or applications. If you would like to test this antibody in an untested species and/or application and share your results with us, then I can recommend our Innovators Reward Program.
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