Detection of Viral B18R by Western Blot.
Western blot shows lysates of CHO Chinese hamster ovary cell line either mock transfected or transfected with viral B18R. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Viral B18R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8185) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for B18R at approximately 80-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
(Soluble interferon alpha/beta receptor B18) is a 60-65 kDa protein
encoded by the Vaccinia virus genome and by the genomes of other
orthopoxviruses. Its function represents one of several mechanisms used
by these viruses to evade the host immune response (1, 2). It is known
as B18R in the Western Reserve (WR) strain of Vaccinia but as B19R in
the Copenhagen strain (3). There is a structurally-unrelated, larger
Vaccinia protein that is also known as B18R (or B16R) that contains
multiple ankyrin-like repeats (4). The soluble interferon receptor B18R,
however, contains three immunoglobulin-like domains and shows homology
to human, mouse, and bovine type I interferon receptors (5). The Wyeth
strain of Vaccinia virus encodes a truncated protein that lacks the
C-terminal Ig-like domain, and B18R is functionally absent in the Lister
strain (6, 7). B18R functions as a decoy receptor for type I
interferons (IFN alpha, beta, omega). It binds to type I interferons
from multiple species and prevents IFN signaling through its receptors
(6-8). B18R binds to the surface of virus infected and uninfected cells
where it retains its capacity to bind and neutralize IFN (6, 8). It
shields those cells from the antiviral effects of type I interferons,
thereby enabling virus replication and pathogenicity (6-8). B18R also
limits the effectiveness of IFN alpha produced following TLR activation
(9), and it limits adaptive T cell responses (3). B18R is a common factor used for increasing the efficiency of RNA reprogramming in induced pluripotent stem cells (IPS cells) (10).
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Perdiguero, B. and M. Esteban (2009) J. Interferon Cytokine Res. 29:581.
Gomez, C.E. et al. (2012) J. Virol. 86:5026.
Goebel, S.J. et al. (1990) Virology 179:247.
Smith, G.L. and Y.S. Chan (1991) J. Gen. Virol. 72:511.
Alcami, A. et al. (2000) J. Virol. 74:11230.
Symons, J.A. et al. (1995) Cell 81:551.
Colamonici, O.R. et al. (1995) J. Biol. Chem. 270:15974.
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