VISTA/B7-H5/PD-1H Antibody (BLR035F)
Novus Biologicals | Catalog # NBP2-76406
Recombinant Monoclonal Antibody
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Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human
Applications
Multiplex Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, COMET
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # BLR035F
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Product Specifications
Immunogen
Immunogen sequence between amino acids 250 and 311 (C-Term)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for VISTA/B7-H5/PD-1H Antibody (BLR035F)
Detection of VISTA in Human Spleen via seqIF™ staining on COMET™
VISTA was detected in immersion fixed paraffin-embedded sections of human Spleen using Rabbit Anti-Human VISTA Recombinant Monoclonal Antibody (Catalog # NBP2-76406) at 1:250 at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.
![Western Blot: VISTA/B7-H5/PD-1H Antibody (BLR035F) [NBP2-76406]](https://resources.rndsystems.com/images/products/VISTA-B7-H5-PD-1H-Antibody-BLR035F-Western-Blot-NBP2-76406-img0003.jpg "Western Blot: VISTA/B7-H5/PD-1H Antibody (BLR035F) [NBP2-76406]")
Western Blot: VISTA/B7-H5/PD-1H Antibody (BLR035F) [NBP2-76406]
Western Blot: VISTA/B7-H5/PD-1H Antibody (BLR035F) [NBP2-76406] - Detection of human VISTA by WB of Jurkat, MOLT-4, MJ, RPMI-8226, HeLa, KG-1, HEK293T, and SUP-M2 lysate. Antibody: Rabbit anti-VISTA recombinant monoclonal [BLR035F] (NBP2-76406). Secondary: HRP-conjugated goat anti-rabbit IgG.Lower Panel: Rabbit anti-GAPDH.![Immunocytochemistry/ Immunofluorescence: VISTA/B7-H5/PD-1H Antibody (BLR035F) [NBP2-76406]](https://resources.rndsystems.com/images/products/VISTA-B7-H5-PD-1H-Antibody-BLR035F-Immunocytochemistry-Immunofluorescence-NBP2-76406-img0004.jpg "Immunocytochemistry/ Immunofluorescence: VISTA/B7-H5/PD-1H Antibody (BLR035F) [NBP2-76406]")
Immunocytochemistry/ Immunofluorescence: VISTA/B7-H5/PD-1H Antibody (BLR035F) [NBP2-76406]
Immunocytochemistry/Immunofluorescence: VISTA/B7-H5/PD-1H Antibody (BLR035F) [NBP2-76406] - Detection of human VISTA (red) in FFPE ovarian carcinoma by IHC-IF. Antibody: Rabbit anti-VISTA recombinant monoclonal [BLR035F] NBP2-76406. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).![Immunohistochemistry-Paraffin: VISTA/B7-H5/PD-1H Antibody (BLR035F) [NBP2-76406]](https://resources.rndsystems.com/images/products/VISTA-B7-H5-PD-1H-Antibody-BLR035F-Immunohistochemistry-NBP2-76406-img0002.jpg "Immunohistochemistry-Paraffin: VISTA/B7-H5/PD-1H Antibody (BLR035F) [NBP2-76406]")
Immunohistochemistry-Paraffin: VISTA/B7-H5/PD-1H Antibody (BLR035F) [NBP2-76406]
Immunohistochemistry-Paraffin: VISTA/B7-H5/PD-1H Antibody (BLR035F) [NBP2-76406] - Detection of human VISTA in FFPE lung carcinoma by IHC. Antibody: Rabbit anti-VISTA recombinant monoclonal [BLR035F] (NBP2-76406). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.![Flow Cytometry: VISTA/B7-H5/PD-1H Antibody (BLR035F) [NBP2-76406]](https://resources.rndsystems.com/images/products/VISTA-B7-H5-PD-1H-Antibody-BLR035F-Flow-Cytometry-NBP2-76406-img0007.jpg "Flow Cytometry: VISTA/B7-H5/PD-1H Antibody (BLR035F) [NBP2-76406]")
Flow Cytometry: VISTA/B7-H5/PD-1H Antibody (BLR035F) [NBP2-76406]
Flow Cytometry: VISTA/B7-H5/PD-1H Antibody (BLR035F) [NBP2-76406] - Rabbit anti-VISTA recombinant monoclonal [BLR035F] (right) or isotype control (left). Secondary: DyLight® 488-conjugated goat anti-rabbit IgG.![Immunocytochemistry/ Immunofluorescence: VISTA/B7-H5/PD-1H Antibody (BLR035F) [NBP2-76406]](https://resources.rndsystems.com/images/products/VISTA-B7-H5-PD-1H-Antibody-BLR035F-Immunocytochemistry-NBP2-76406-img0001.jpg "Immunocytochemistry/ Immunofluorescence: VISTA/B7-H5/PD-1H Antibody (BLR035F) [NBP2-76406]")
Immunocytochemistry/ Immunofluorescence: VISTA/B7-H5/PD-1H Antibody (BLR035F) [NBP2-76406]
Immunocytochemistry/Immunofluorescence: VISTA/B7-H5/PD-1H Antibody (BLR035F) [NBP2-76406] - Detection of human VISTA in FFPE NCI-H226 cells by ICC. Antibody: Rabbit anti-VISTA recombinant monoclonal [BLR035F] (NBP2-76406). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.![Immunoprecipitation: VISTA/B7-H5/PD-1H Antibody (BLR035F) [NBP2-76406]](https://resources.rndsystems.com/images/products/VISTA-B7-H5-PD-1H-Antibody-BLR035F-Immunoprecipitation-NBP2-76406-img0006.jpg "Immunoprecipitation: VISTA/B7-H5/PD-1H Antibody (BLR035F) [NBP2-76406]")
Immunoprecipitation: VISTA/B7-H5/PD-1H Antibody (BLR035F) [NBP2-76406]
Immunoprecipitation: VISTA/B7-H5/PD-1H Antibody (BLR035F) [NBP2-76406] - Detection of human VISTA by WB of immunoprecipitates from Jurkat lysate. Antibodies: Rabbit anti-VISTA recombinant monoclonal [BLR035F] (NBP2-76406) and another rabbit anti-VISTA recombinant monoclonal.Applications for VISTA/B7-H5/PD-1H Antibody (BLR035F)
Application
Recommended Usage
Flow Cytometry
Fixed in 4% formaldehyde and permeabilized with 90% methanol. 2 µl
Immunocytochemistry/ Immunofluorescence
1:100 - 1:500
Immunohistochemistry
1:100 - 1:500
Immunohistochemistry-Paraffin
1:100 - 1:500
Immunoprecipitation
20 ul/1mg lysate
Multiplex Immunofluorescence
1:250
Western Blot
1:1000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 2 - 8 C / 1 year from date of receipt
Background: VISTA/B7-H5/PD-1H
Alternate Names
4632428N05Rik, B7-H5, C10orf54, Dies1, Gi24, PD-1H, PD1H, SISP1, VSIR
Gene Symbol
VSIR
Additional VISTA/B7-H5/PD-1H Products
Product Documents for VISTA/B7-H5/PD-1H Antibody (BLR035F)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for VISTA/B7-H5/PD-1H Antibody (BLR035F)
This Product sold under License from Bethyl Laboratories, Inc.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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