VPS33A Antibody - BSA Free

Immunohistochemistry-Paraffin: VPS33A Antibody [NBP2-20872]

Immunohistochemistry-Paraffin: VPS33A Antibody [NBP2-20872] - Immunohistochemical analysis of paraffin-embedded colon ca, using antibody at 1:500 dilution.

Western Blot: VPS33A Antibody [NBP2-20872]

VPS33A-Antibody-Knockdown-Validated-NBP2-20872-img0007.jpg

Western Blot: VPS33A Antibody [NBP2-20872] -

Western Blot: VPS33A Antibody [NBP2-20872] - VPS33A was the direct target of miR-423-5p. (A) The location of the two miR-423-5p binding domains. Interaction domains are indicated by red font. Mutant sequences are indicated by bold font. (B) The structure diagram of the constructed plasmids. (C) A 2 × 4 (factor of miRNA: NC mimic & miR-423-5p mimic, 100 nM; factor of luciferase reporter plasmid: pmirGLO vector (mock), pmirGLO-WT, pmirGLO-MT1, pmirGLO-MT2 & pmirGLO-MT (1 + 2)) orthogonal experiment for Dual-Glo-Luciferase-Assay was performed. (D) The co-localization of LC3-GFP puncta with lysosomes in the macrophages transfected with VPS33A specific siRNA (negative siRNA as NC) was analyzed. More than 20 cells from three independent experiments were analyzed. Scale bars: 5 μm. (E) The expression levels of VPS33A in the macrophages transfected with VPS33A specific siRNA or NC siRNA were analyzed. (F) The co-localization of LC3-GFP puncta with lysosomes in the macrophages transfected with NC mimic, miR-423-5p mimic, or miR-423-5p mimic + pcDNA-VPS33A were analyzed. More than 20 cells from three independent experiments were analyzed. Scale bars: 5 μm. (G) The protein levels of VPS33A in the macrophages transfected with NC mimic, miR-423-5p mimic, or miR-423-5p mimic + pcDNA-VPS33A were analyzed by western blot. The statistical results were obtained from more than three independent experiments. Target genes were normalized with beta -actin. Data are presented as mean ± SD, & Error bars indicate the SD. NC: negative control. *P < 0.05, ** P < 0.01, *** P < 0.001. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30898038), licensed under a CC-BY license. Not internally tested by Novus Biologicals.