WDHD1 Antibody - Azide and BSA Free

Western Blot: WDHD1 Antibody - Azide and BSA Free [NBP2-93674] -

DNA demethylases TET1/TET3 activates WDHD1 in LUAD cells. (A) The level of WDHD1 promoter methylation in LUAD cells (H1944, A549, PC-9, and H1975 cells) and the human bronchial epithelial cell line (BEAS-2B) was examined using qMSP. (B) The protein expression of TET1, TET2, and TET3 in LUAD cells (H1944, A549, PC-9, and H1975 cells) and BEAS-2B cells was examined using western blot analysis. (C) The methylation of the WDHD1 promoter in tumors and paracancerous tissues of 16 LUAD patients was examined using qMSP. (D) The protein expression of TET1 and TET3 in tumors and paracancerous tissues of 16 LUAD patients was examined using western blot analysis. (E) Transcriptional levels of WDHD1, TET1, and TET3 in A549 and PC-9 cells after infection with KD-TET1 or KD-TET3 were examined using RT-qPCR. (F) The protein expression of WDHD1, TET1, and TET3 in LUAD cells after infection with KD-TET1 or KD-TET3 was examined using western blot analysis. (G) The level of WDHD1 promoter methylation in A549 and PC-9 cells after infection with KD-TET1 or KD-TET3 was examined using qMSP. p-values were calculated using paired t-tests or two-way ANOVA. Mean +/- SEM was presented. The average results from five independent experiments are shown Image collected and cropped by CiteAb from the following open publication (https://respiratory-research.biomedcentral.com/articles/10.1186/s12931-…), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: WDHD1 Antibody - Azide and BSA Free [NBP2-93674] -

Exogenous expression of WDHD1 reverses knockdown of TET1/TET3-blocked LUAD glycolysis. (A) Transcriptional levels of WDHD1 in A549 and PC-9 cells after infection of KD-TET1 + OE-WDHD1 or KD-TET3 + OE-WDHD1 were examined using RT-qPCR. (B) The protein expression of WDHD1 in LUAD cells after infection of KD-TET1 + OE-WDHD1 or KD-TET3 + OE-WDHD1 was examined using western blot analysis. Glycolytic activity and capacity of A549 (C) and PC-9 (D) cells were analyzed. (E) Detection of 2,3-BPG contents in A549 and PC-9 cells after infection of KD-TET1 or KD-TET3 alone or combined with OE-WDHD1 by ELISA. (F) Detection of 2-PG in A549 and PC-9 cells after infection of KD-TET1 or KD-TET3 alone or combined with OE-WDHD1 by ELISA. (G) Lactate production in A549 and PC-9 cells after infection of KD-TET1 or KD-TET3 alone or combined with OE-WDHD1. p-values were calculated using two-way ANOVA. Mean +/- SEM was presented. The average results from five independent experiments are shown Image collected and cropped by CiteAb from the following open publication (https://respiratory-research.biomedcentral.com/articles/10.1186/s12931-…), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: WDHD1 Antibody - Azide and BSA Free [NBP2-93674] -

DNA demethylases TET1/TET3 activates WDHD1 in LUAD cells. (A) The level of WDHD1 promoter methylation in LUAD cells (H1944, A549, PC-9, and H1975 cells) and the human bronchial epithelial cell line (BEAS-2B) was examined using qMSP. (B) The protein expression of TET1, TET2, and TET3 in LUAD cells (H1944, A549, PC-9, and H1975 cells) and BEAS-2B cells was examined using western blot analysis. (C) The methylation of the WDHD1 promoter in tumors and paracancerous tissues of 16 LUAD patients was examined using qMSP. (D) The protein expression of TET1 and TET3 in tumors and paracancerous tissues of 16 LUAD patients was examined using western blot analysis. (E) Transcriptional levels of WDHD1, TET1, and TET3 in A549 and PC-9 cells after infection with KD-TET1 or KD-TET3 were examined using RT-qPCR. (F) The protein expression of WDHD1, TET1, and TET3 in LUAD cells after infection with KD-TET1 or KD-TET3 was examined using western blot analysis. (G) The level of WDHD1 promoter methylation in A549 and PC-9 cells after infection with KD-TET1 or KD-TET3 was examined using qMSP. p-values were calculated using paired t-tests or two-way ANOVA. Mean +/- SEM was presented. The average results from five independent experiments are shown Image collected and cropped by CiteAb from the following open publication (https://respiratory-research.biomedcentral.com/articles/10.1186/s12931-…), licensed under a CC-BY license. Not internally tested by Novus Biologicals.