WDR79 Antibody

Novus Biologicals | Catalog # NB100-68252

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Chemotaxis

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all WDR79 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human WD repeat domain 79 using the numbering given in entry NP_060551.1 (GeneID 55135).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for WDR79 Antibody

Western Blot: WDR79 Antibody [NB100-68252]

Western Blot: WDR79 Antibody [NB100-68252]

WDR79-Antibody-Western-Blot-NB100-68252-img0007.jpg
Immunohistochemistry: WDR79 Antibody [NB100-68252]

Immunohistochemistry: WDR79 Antibody [NB100-68252]

WDR79-Antibody-Immunohistochemistry-NB100-68252-img0008.jpg
Western Blot: WDR79 Antibody [NB100-68252]

Western Blot: WDR79 Antibody [NB100-68252]

Western Blot: WDR79 Antibody [NB100-68252] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, K-562, and U2OS cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-WDR79 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Western Blot: WDR79 Antibody [NB100-68252]

Western Blot: WDR79 Antibody [NB100-68252]

WDR79-Antibody-Western-Blot-NB100-68252-img0006.jpg
Immunohistochemistry-Paraffin: WDR79 Antibody [NB100-68252]

Immunohistochemistry-Paraffin: WDR79 Antibody [NB100-68252]

Immunohistochemistry-Paraffin: WDR79 Antibody [NB100-68252] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti-WDR79 used at a dilution of 1:200 (1ug/ml). Detection: DAB
Immunohistochemistry: WDR79 Antibody [NB100-68252]

Immunohistochemistry: WDR79 Antibody [NB100-68252]

WDR79-Antibody-Immunohistochemistry-NB100-68252-img0009.jpg
Immunoprecipitation: WDR79 Antibody [NB100-68252]

Immunoprecipitation: WDR79 Antibody [NB100-68252]

Immunoprecipitation: WDR79 Antibody [NB100-68252] - Detection of human WDR79 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-WDR79 antibody NB100-68252 (lot NB100-68252-2) used for IP at 6 ug per reaction. WDR79 was also immunoprecipitated by a previous lot of this antibody (lot NB100-68252-1). For blotting immunoprecipitated WDR79, NB100-68252 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for WDR79 Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Immunocytochemistry/ Immunofluorescence

1:50 -1:250

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
KO Validation was reported in scientific literature (PMID: 27525486). ICC/IF reactivity reported in (PMID: 27525486), Chip reactivity reported in (PMID: 22547674). Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Reviewed Applications

Read 1 review rated 5 using NB100-68252 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: WDR79

WD-repeat domain 79 (WDR79) contains six WD (tryptophan-aspartate) repeat domains found in a number of proteins that function as adaptor molecules in signal transduction and cytoskeletal organization. The WD repeat is defined by four or more repeating units of a conserved core of approximately 40 amino acids ending with tryptophan-aspartic acid (WD). WD repeats may serve as sites of protein-protein interaction for adaptor proteins and facilitate multiprotein complex formation. The function of the WDR79 protein has not been characterized, however significant and consistent single nucleotide polymorphisms in the WDR79 gene have been found to be associated with ER negative breast cancer.

Alternate Names

FLJ10385, TCAB1WDR79telomerase Cajal body protein 1, WD repeat containing, antisense to TP53, WD repeat domain 79, WD repeat-containing protein 79, WD40 protein Wrap53, WD40 repeat-containing protein encoding RNA antisense to p53, WD-encoding RNA antisense to p53

Entrez Gene IDs

55135 (Human)

Gene Symbol

WRAP53

UniProt

Q9BUR4 (Human)

Additional WDR79 Products

Product Documents for WDR79 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for WDR79 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for WDR79 Antibody (1)

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Customer Images

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  • Name: I Sawyer
    Application: Immunofluorescence
    Sample Tested: hela
    Species: Human
    Verified Customer | Posted 02/26/2016
    Co-localization of WDR79 with coilin in HeLa cells
    WDR79 Antibody NB100-68252

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Protocols

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