Wnt-10b Antibody (HL2370) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25749

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat, Zebrafish

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2370 expressed in HEK293

Format

Azide and BSA Free
View all Wnt-10b Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human Wnt-10b.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Wnt-10b Antibody (HL2370) - Azide and BSA Free

Wnt-10b Antibody (HL2370) - Azide and BSA Free

Immunohistochemistry-Paraffin: Wnt-10b Antibody (HL2370) - Azide and BSA Free [NBP3-25749] -

Immunohistochemistry-Paraffin: Wnt-10b Antibody (HL2370) - Azide and BSA Free [NBP3-25749] - Wnt10b antibody [HL2370] detects Wnt10b protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. Wnt10b stained by Wnt10b antibody [HL2370] (NBP3-25749) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Wnt-10b Antibody (HL2370) - Azide and BSA Free

Western Blot: Wnt-10b Antibody (HL2370) - Azide and BSA Free [NBP3-25749] -

Western Blot: Wnt-10b Antibody (HL2370) - Azide and BSA Free [NBP3-25749] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt10b antibody [HL2370] (NBP3-25749) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Wnt-10b Antibody (HL2370) - Azide and BSA Free

Western Blot: Wnt-10b Antibody (HL2370) - Azide and BSA Free [NBP3-25749] -

Western Blot: Wnt-10b Antibody (HL2370) - Azide and BSA Free [NBP3-25749] - Whole zebrafish extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt10b antibody [HL2370] (NBP3-25749) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Wnt-10b Antibody (HL2370) - Azide and BSA Free

Immunohistochemistry-Paraffin: Wnt-10b Antibody (HL2370) - Azide and BSA Free [NBP3-25749] -

Immunohistochemistry-Paraffin: Wnt-10b Antibody (HL2370) - Azide and BSA Free [NBP3-25749] - Wnt10b antibody [HL2370] detects Wnt10b protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. Wnt10b stained by Wnt10b antibody [HL2370] (NBP3-25749) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Wnt-10b Antibody (HL2370) - Azide and BSA Free

Western Blot: Wnt-10b Antibody (HL2370) - Azide and BSA Free [NBP3-25749] -

Western Blot: Wnt-10b Antibody (HL2370) - Azide and BSA Free [NBP3-25749] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt10b antibody [HL2370] (NBP3-25749) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Wnt-10b Antibody (HL2370) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Wnt-10b Antibody (HL2370) [NBP3-25749] -

Wnt-10b antibody [HL2370] detects Wnt-10b protein at cell membrane by immunofluorescent analysis.
Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Wnt-10b stained by Wnt-10b antibody [HL2370] (NBP3-25749) diluted at 1:500.
Blue: Fluoroshield with DAPI.

Applications for Wnt-10b Antibody (HL2370) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

Assay dependent

Immunohistochemistry-Paraffin

Assay dependent

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Wnt-10b

Researchers have indicated that they want access to novel target antibodies quickly to aid in their research. Novus is pleased to offer researchers access to products that are not fully characterized or validated. This antibody is shown by Peptide ELISA to bind to the peptide used as immunogen. Investigators should empirically determine the suitability of the antibody, including optimal dilutions, for other applications of interest. * We cannot guarantee that this antibody will work in any application other than in a Peptide ELISA against the peptide used as immunogen and therefore can not offer a refund if the antibody does not work in your application. * The antibody is offered at a lower price compared to more highly validated antibodies. * We are not able to provide the peptide used as immunogen for this product. As this antibody is not fully validated we appreciate your feedback. Customer feedback is used to help generate testing methodologies which can lead to further product validation or withdrawal.__________________________ Wnts comprise a family of secreted signaling proteins that regulate diverse developmental processes. Activation of Wnt signaling by Wnt10b inhibits differentiation of preadipocytes and blocks adipose tissue development. In bone development, Wnt10b shifts cell fate toward the osteoblast lineage.

Long Name

Wingless-type MMTV Integration Site Family, Member 10B

Alternate Names

Wnt-12, Wnt10b

Gene Symbol

WNT10B

Additional Wnt-10b Products

Product Documents for Wnt-10b Antibody (HL2370) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Wnt-10b Antibody (HL2370) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Wnt Ligands (Wnts)

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Protocols

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FAQs

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