WT1 Antibody (6F-H2) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-81012

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 6F-H2

Format

Azide and BSA Free
View all WT1 Primary Antibodies »

Product Specifications

Immunogen

Human recombinant Wilms Tumor 1 protein (WT1) (residues 1-181). [Swiss-Prot: P19544]

Localization

Nuclear, Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for WT1 Antibody (6F-H2) - Azide and BSA Free

Western Blot: WT1 Antibody (6F-H2)Azide and BSA Free [NBP2-81012]

Western Blot: WT1 Antibody (6F-H2)Azide and BSA Free [NBP2-81012]

Western Blot: WT1 Antibody (6F-H2) - Azide and BSA Free [NBP2-81012] - Whole cell protein from human K562, HEK293 and kidney tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-WT1 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.
Immunohistochemistry: WT1 Antibody (6F-H2) - Azide and BSA Free [NBP2-81012]

Immunohistochemistry: WT1 Antibody (6F-H2) - Azide and BSA Free [NBP2-81012]

Immunohistochemistry: WT1 Antibody (6F-H2) - Azide and BSA Free [NBP2-81012] - Staining of human kidney glomerulus tissue. Heat mediated antigen retrieval was performed by heating in citrate buffer (pH 6) at 95C for 20 minutes. Image from verified customer review. Image from the standard format of this antibody.
Western Blot: WT1 Antibody (6F-H2)Azide and BSA Free [NBP2-81012]

Western Blot: WT1 Antibody (6F-H2)Azide and BSA Free [NBP2-81012]

Western Blot: WT1 Antibody (6F-H2) - Azide and BSA Free [NBP2-81012] - Hypomethylation of the Intron 1 CpG island results in WT1 expression. RNA was isolated from K562 and U937 cells growing under atmospheric conditions (Normox) and from U937 cells growing in 1% O2 (Hypox) and was analyzed by RT-PCR using primers that span exon 5 of the WT1 mRNA. Ribosomal RNA 36B4 is used as a positive control, and molecular weight markers (MW) are shown. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0119837), licensed under a CC-BY license. Image from the standard format of this antibody.
Immunohistochemistry: WT1 Antibody (6F-H2) - Azide and BSA Free [NBP2-81012]

Immunohistochemistry: WT1 Antibody (6F-H2) - Azide and BSA Free [NBP2-81012]

Immunohistochemistry: WT1 Antibody (6F-H2) - Azide and BSA Free [NBP2-81012] - Analysis of Wilms Tumor 1 in human renal cancer using DAB with hematoxylin counterstain. Image from the standard format of this antibody.
Simple Western: WT1 Antibody (6F-H2)Azide and BSA Free [NBP2-81012]

Simple Western: WT1 Antibody (6F-H2)Azide and BSA Free [NBP2-81012]

Simple Western: WT1 Antibody (6F-H2) - Azide and BSA Free [NBP2-81012] - Lane view shows a specific band for WT1 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image from the standard format of this antibody.

Applications for WT1 Antibody (6F-H2) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 25576053)

Immunohistochemistry

1:400

Immunohistochemistry-Frozen

1:400

Immunohistochemistry-Paraffin

1:400

Immunoprecipitation

2-10 ug/ml lysate

Simple Western

1:50

Western Blot

2 ug/ml
Application Notes
In Western blot, a doublet is observed at approx. 55 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In immunostaining, this target may be found localized to nucleus/nucleolus and cytoplasm of cells (WT1 shuttles between nucleus and cytoplasm - PMID: 14681305). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: antibody dilution of 1:50. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: WT1

Wilms' tumor 1 (WT1) gene inactivation is responsible for almost 20% of Wilms' tumour/developmental renal tumour or nephroblastoma cases and besides playing important role in cellular development and survival, this homodimeric transcription factor has been shown to involve in certain pathologies also. WT1 belongs to EGR C2H2-type zinc-finger protein family and interacts with several proteins including WTIP, HNRNPU, U2AF2, CITED2, ZNF224, WTAP, SRY, AMER1, RBM4 and actively translating polysomes (detected in nuclear ribonucleoprotein particles) for exerting its activities. WT1 binds to DNA sequence 5'-CGCCCCCGC-3' and regulates the expression of several target genes including EPO. It plays essential role in urogenital system development and in carcinogenesis, it has been suggested as tumor suppressor as well as oncogenic protein. Defects in WT1 have been linked to Wilms tumor 1 (WT1), Frasier syndrome (FS), Denys-Drash syndrome (DDS), nephrotic syndrome type 4 (NPHS4), Meacham syndrome (MEACHS), mesothelioma malignant (MESOM).

Long Name

Wilms Tumor 1

Alternate Names

GUD, WAGR, WIT-2, WT33

Gene Symbol

WT1

Additional WT1 Products

Product Documents for WT1 Antibody (6F-H2) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for WT1 Antibody (6F-H2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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