WWP1 Antibody (1A7) - Azide and BSA Free

Western Blot: WWP1 Antibody (1A7) [H00011059-M01]

Western Blot: WWP1 Antibody (1A7) [H00011059-M01] - WWP1 expression in A-431 ( Cat # L015V1 ).

Immunoprecipitation: WWP1 Antibody (1A7) [H00011059-M01]

Immunoprecipitation: WWP1 Antibody (1A7) [H00011059-M01] - Analysis of WWP1 transfected lysate using anti-WWP1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with WWP1 MaxPab rabbit polyclonal antibody.

ELISA: WWP1 Antibody (1A7) [H00011059-M01]

ELISA: WWP1 Antibody (1A7) [H00011059-M01] - Detection limit for recombinant GST tagged WWP1 is 0.03 ng/ml as a capture antibody.

Western Blot: WWP1 Antibody (1A7) - Azide and BSA Free [H00011059-M01] -

High cell density promotes WWP1 stabilization and activation.(A) RPE-1 cells were stably transduced with FLAG-AMOTL2 and either control or WWP1 shRNAs, re-seeded to a sparse or confluent density, then subjected to in vivo ubiquitination assays. (B) mRNAs isolated from MCF10A and 293Ad cells, seeded at either a sparse or confluent density, were analyzed for the indicated genes by qRT-PCR, and expression levels were normalized to those of GAPDH mRNA (n = 4). Data are expressed as means +/- SEM (error bars; ***P < 0.001, n.s. not significant; unpaired t test). (C) MCF10A cells stably transduced with shGFP (control) or shWWP1 lentiviruses were seeded at either a sparse or confluent density and extracts were analyzed by Western blotting. (D) RPE-1 and 293Ad cells were seeded at either a sparse or confluent density, then a cycloheximide (CHX) chase assay was performed for the indicated times. The resulting extracts were analyzed by Western blotting. (E) 293Ad cells were seeded at either sparse or confluent densities and extracts were subjected to S100/P100 membrane-cytosol fractionation assays. The resulting fractions were analyzed for the indicated proteins by Western blotting. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34404733), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: WWP1 Antibody (1A7) - Azide and BSA Free [H00011059-M01] -

High cell density promotes WWP1 stabilization and activation.(A) RPE-1 cells were stably transduced with FLAG-AMOTL2 and either control or WWP1 shRNAs, re-seeded to a sparse or confluent density, then subjected to in vivo ubiquitination assays. (B) mRNAs isolated from MCF10A and 293Ad cells, seeded at either a sparse or confluent density, were analyzed for the indicated genes by qRT-PCR, and expression levels were normalized to those of GAPDH mRNA (n = 4). Data are expressed as means +/- SEM (error bars; ***P < 0.001, n.s. not significant; unpaired t test). (C) MCF10A cells stably transduced with shGFP (control) or shWWP1 lentiviruses were seeded at either a sparse or confluent density and extracts were analyzed by Western blotting. (D) RPE-1 and 293Ad cells were seeded at either a sparse or confluent density, then a cycloheximide (CHX) chase assay was performed for the indicated times. The resulting extracts were analyzed by Western blotting. (E) 293Ad cells were seeded at either sparse or confluent densities and extracts were subjected to S100/P100 membrane-cytosol fractionation assays. The resulting fractions were analyzed for the indicated proteins by Western blotting. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34404733), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: WWP1 Antibody (1A7) - Azide and BSA Free [H00011059-M01] -

High cell density promotes WWP1 stabilization and activation.(A) RPE-1 cells were stably transduced with FLAG-AMOTL2 and either control or WWP1 shRNAs, re-seeded to a sparse or confluent density, then subjected to in vivo ubiquitination assays. (B) mRNAs isolated from MCF10A and 293Ad cells, seeded at either a sparse or confluent density, were analyzed for the indicated genes by qRT-PCR, and expression levels were normalized to those of GAPDH mRNA (n = 4). Data are expressed as means +/- SEM (error bars; ***P < 0.001, n.s. not significant; unpaired t test). (C) MCF10A cells stably transduced with shGFP (control) or shWWP1 lentiviruses were seeded at either a sparse or confluent density and extracts were analyzed by Western blotting. (D) RPE-1 and 293Ad cells were seeded at either a sparse or confluent density, then a cycloheximide (CHX) chase assay was performed for the indicated times. The resulting extracts were analyzed by Western blotting. (E) 293Ad cells were seeded at either sparse or confluent densities and extracts were subjected to S100/P100 membrane-cytosol fractionation assays. The resulting fractions were analyzed for the indicated proteins by Western blotting. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34404733), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: WWP1 Antibody (1A7) - Azide and BSA Free [H00011059-M01] -

High cell density promotes WWP1 stabilization and activation.(A) RPE-1 cells were stably transduced with FLAG-AMOTL2 and either control or WWP1 shRNAs, re-seeded to a sparse or confluent density, then subjected to in vivo ubiquitination assays. (B) mRNAs isolated from MCF10A and 293Ad cells, seeded at either a sparse or confluent density, were analyzed for the indicated genes by qRT-PCR, and expression levels were normalized to those of GAPDH mRNA (n = 4). Data are expressed as means +/- SEM (error bars; ***P < 0.001, n.s. not significant; unpaired t test). (C) MCF10A cells stably transduced with shGFP (control) or shWWP1 lentiviruses were seeded at either a sparse or confluent density and extracts were analyzed by Western blotting. (D) RPE-1 and 293Ad cells were seeded at either a sparse or confluent density, then a cycloheximide (CHX) chase assay was performed for the indicated times. The resulting extracts were analyzed by Western blotting. (E) 293Ad cells were seeded at either sparse or confluent densities and extracts were subjected to S100/P100 membrane-cytosol fractionation assays. The resulting fractions were analyzed for the indicated proteins by Western blotting. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34404733), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: WWP1 Antibody (1A7) - Azide and BSA Free [H00011059-M01] -

WWP1 promotes AMOTL2 phosphorylation by LATS.(A) 293T cells stably transduced with shGFP (control) or shWWP1 lentiviruses were transfected with the indicated DNAs, then lysates were analyzed by Western blotting. (B) 293T cells were transfected with the indicated DNAs, then lysates were analyzed by Western blotting. (C) 293T cells were transfected with the indicated DNAs, then lysates were analyzed by Western blotting. (D) Control or CRISPR/Cas9-mediated LATS1/2-knockout 293T cells were transfected with the indicated DNAs, then lysates were subjected to in vivo ubiquitination assay. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34404733), licensed under a CC-BY license. Not internally tested by Novus Biologicals.