XIAP Antibody - BSA Free

Novus Biologicals | Catalog # NB100-56183

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all XIAP Primary Antibodies »

Product Specifications

Immunogen

Recombinant BIR2 domain protein fragment of human XIAP was used as immunogen. The BIR2 domain used for immunogen corresponds to amino acids 163-230 of human XIAP (Deveraux et al, 1999).

Specificity

The antibody recognizes epitopes in the BIR2 domain of XIAP. Therefore it can recognize full-length XIAP and XIAP cleavage fragments containing the BIR2 domain. However, XIAP cleavage fragments may be biologically unstable, and therefore cleavage fragments may be difficult to detect.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for XIAP Antibody - BSA Free

Western Blot: XIAP Antibody [NB100-56183]

Western Blot: XIAP Antibody [NB100-56183]

Western Blot: XIAP Antibody - Unpurified [NB100-56183] - Analysis of XIAP in various tumor cell lines using this antibody at 1:2000.
Immunocytochemistry/ Immunofluorescence: XIAP Antibody [NB100-56183]

Immunocytochemistry/ Immunofluorescence: XIAP Antibody [NB100-56183]

XIAP-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56183-img0007.jpg
Immunohistochemistry-Paraffin: XIAP Antibody [NB100-56183]

Immunohistochemistry-Paraffin: XIAP Antibody [NB100-56183]

Immunohistochemistry-Paraffin: XIAP Antibody - Unpurified [NB100-56183] - Human breast carcinoma using this antibody at 1:2000. A-A3, successively higher magnifications of the breast carcinoma tissue section. Hematoxylin-eosin counterstain.
Western Blot: XIAP Antibody [NB100-56183]

Western Blot: XIAP Antibody [NB100-56183]

Western Blot: XIAP Antibody - Unpurified [NB100-56183] - XIAP Antibody [NB100-56183] - Analysis of recombinant full-length IAP proteins using this antibody to XIAP at 1:2000. The data shows that the antibody recognizes only XIAP and not the other IAP proteins.
XIAP Antibody

Immunocytochemistry/ Immunofluorescence: XIAP Antibody [NB100-56183] -

Immunocytochemistry/ Immunofluorescence: XIAP Antibody [NB100-56183] - Kaempferol inhibits the activity of caspase-3 enzyme. (a & b) Caspase-3 inhibition by kaempferol was measured using recombinant caspase-3 (enzyme) & Z-DEVD-aminoluciferin (substrate) with respect to time (a) & concentration (b). Ac-DEVD-CHO served as a positive control. (c) Dose response curve for kaempferol inhibition on caspase-3 enzyme activity. (d) Analysis of the inhibitory potential of estrogen receptor modulators on caspase-3 enzyme activity. Withanaloide A served as (−) control & Ac-DEVE-CHO as (+) control. Data represented as mean ± SEM of two independent experiments performed in duplicate. (e) Immunocytochemistry for XIAP expression levels in IMR32 cells after 24 hours of incubation in respective conditions (Scale bar: 67 µm). Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/s41598-018-20499-7), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for XIAP Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 29391535)

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:1000-1:5000

Immunoprecipitation

1:50-1:200

Western Blot

1:1000-1:2000
Application Notes
Although not tested this antibody may be useful in Immunohistochemistry-Frozen.

Reviewed Applications

Read 3 reviews rated 4.3 using NB100-56183 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: XIAP

XIAP [human X-linked IAP, hILP (human IAP-like protein), MIHA, BIRC4) is a member of the family of inhibitor of apoptosis proteins (IAP). IAPs suppress mitochondria-dependent and independent apoptosis by binding to and inhibiting caspases through their BIR domains (reviewed in Liston et al, 2003; Wright and Duckett, 2005). Resistance towards apoptosis is a hallmark of cancer cells, and overexpression of IAPs can contribute to the development of cancer though inhibiting apoptosis. In addition to at least one BIR domain, some IAP members also have a RING-type finger motif at their carboxyl-terminal. The RING finger domain of several IAPs, including XIAP, have E3 ubiquitin ligase activity and target the degradation of Smac/DIABLO through ubiquitination (Morizane et al, 2005). Smac/DIABLO is a death inducer and functions by inhibiting IAP-caspase interactions, thereby promoting apoptosis. Degradation of cell death inducers like Smac/DIABLO is thought to be a conserved mechanism by which IAPs enhance their anti-apoptotic activity, thereby promoting cell survival. XIAP is highly characterized with respect to its structure and biochemical mechanisms and has received interest as a therapeutic target (reviewed in Schimmer, 2006). Since XIAP blocks a substantial portion of the apoptosis pathway and is associated with chemoresistance in cancer cells, inhibiting XIAP has been a focus for potential therapeutics. Approaches have included antisense oligonucleotides and small molecule inhibitors. Small molecules that that target the BIR2 and BIR3 domains of XIAP are considered particularly attractive. This is because the BIR domains inhibit caspase activity, and it is thought that removing the inhibition should increase the cell's ability to undergo apoptosis as well as decrease its potential for chemoresistance. Full-length human XIAP is a 497 amino acid protein and migrates at approx. 53 kDa on SDS-PAGE.

Long Name

X-linked Inhibitor of Apoptosis

Alternate Names

BIRC4

Entrez Gene IDs

331 (Human); 63879 (Rat)

Gene Symbol

XIAP

Additional XIAP Products

Product Documents for XIAP Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for XIAP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cysteine Proteases and Regulators

Citations for XIAP Antibody - BSA Free

Customer Reviews for XIAP Antibody - BSA Free (3)

4.3 out of 5
3 Customer Ratings
5 Stars
67%
4 Stars
0%
3 Stars
33%
2 Stars
0%
1 Stars
0%

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Showing  1 - 3 of 3 reviews Showing All
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  • XIAP Antibody
    Name: PRAVEEN Dubey
    Application: Western Blot
    Sample Tested: H9c2 whole cell lysate
    Species: Rat
    Verified Customer | Posted 02/07/2022
    XIAP
    XIAP Antibody - BSA Free NB100-56183
  • XIAP Antibody
    Name: Anonymous
    Application: Immunoprecipitation
    Sample Tested: AC16
    Species: Human
    Verified Customer | Posted 04/20/2021
    XIAP
    XIAP Antibody - BSA Free NB100-56183
  • XIAP Antibody
    Name: Anonymous
    Application: Immunoprecipitation
    Sample Tested: AC16
    Species: Human
    Verified Customer | Posted 05/17/2019
    Excellent Antibody
    XIAP Antibody - BSA Free NB100-56183

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