Ymer Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88265

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Ymer Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QEKKDEDIARLLQEKELQEEKKRKKHFPEFPATRAYADSYYYEDGGMKPRVMKEAVSTPSRMAHRDQEWYDAEIARKLQEEELLATQVDMRAAQVAQDEEIARLLMAEEKKAYKKAKEREKSSLDKRKQDPEWKPKTAKAANSKSKESDE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Ymer Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Ymer Antibody [NBP1-88265]

Immunocytochemistry/ Immunofluorescence: Ymer Antibody [NBP1-88265]

Immunocytochemistry/Immunofluorescence: Ymer Antibody [NBP1-88265] - Staining of human cell line U-251 MG shows localization to cytosol.
Immunohistochemistry-Paraffin: Ymer Antibody [NBP1-88265]

Immunohistochemistry-Paraffin: Ymer Antibody [NBP1-88265]

Immunohistochemistry-Paraffin: Ymer Antibody [NBP1-88265] - Staining of human lymph node shows strong cytoplasmic positivity in a subset of lymphoid cells outside reaction center.
Ymer Antibody - BSA Free Western Blot: Ymer Antibody - BSA Free [NBP1-88265]

Western Blot: Ymer Antibody - BSA Free [NBP1-88265]

Analysis in human cell lines U-251MG and A-549 using Anti-CCDC50 antibody. Corresponding CCDC50 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.
Ymer Antibody - BSA Free Western Blot: Ymer Antibody - BSA Free [NBP1-88265]

Western Blot: Ymer Antibody - BSA Free [NBP1-88265]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Applications for Ymer Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ymer

Ymer encodes a soluble, cytoplasmic, tyrosine-phosphorylated protein with multiple ubiquitin-interacting domains. Mutations in this gene cause nonsyndromic, postlingual, progressive sensorineural DFNA44 hearing loss. In mouse, the protein is expressed in the inner ear during development and postnatal maturation and associates with microtubule-based structures. This protein may also function as a negative regulator of NF-kB signaling and as an effector of epidermal growth factor (EGF)-mediated cell signaling. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]

Alternate Names

C3orf6deafness, autosomal dominant 44, coiled-coil domain containing 50, coiled-coil domain-containing protein 50, DFNA44, Protein Ymer, YMER

Gene Symbol

CCDC50

Additional Ymer Products

Product Documents for Ymer Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Ymer Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Ymer Antibody - BSA Free

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Protocols

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