ZBP1/DLM-1/DAI Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: ZBP1/DLM-1/DAI Antibody - BSA Free [NBP1-76854] -

ZBP1 is mainly expressed in macrophages and alters the ratio of M1 and M2 macrophages in sepsis‐induced myocardial dysfunction. A single‐nucleus mRNA sequencing (snRNA‐seq) was performed in hearts from LPS‐treated WT or Zbp1−/− mice (for each group, single‐cell suspensions from three hearts were pooled as one sample). (A) UMAP plot of cell clusters in myocardial tissues from WT and Zbp1−/− mice induced by LPS. CM, cardiomyocyte; EC, endothelial cell; FB, fibroblast; Mac, macrophage; NP, neutrophil. (B,C) UMAP plot and dot plot of Zbp1 expression in myocardial tissues from WT and Zbp1−/− mice induced by LPS. (D,E) UMAP plot of macrophage in myocardial tissues from WT and Zbp1−/− mice induced by LPS. M1, M1 macrophage; M2, M2 macrophage. (F–H) Heart tissues were labelled with M1 macrophage marker iNOS and M2 macrophage marker CD206. Tissues were counterstained with DAPI. Representative staining images (F) and quantification of positive cells (G,H) are shown [scale bar = 50 um, n = 5]. Mean +/- SEM; *P < 0.05, ***P < 0.001, ns, no significance. WT, wild‐type; ZBP1, Z‐DNA binding protein 1; LPS, lipopolysaccharide; snRNA‐seq, single‐nucleus mRNA sequencing. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40289345), licensed under a CC-BY license. Not internally tested by Novus Biologicals.