ZBTB7A/Pokemon Antibody (3T2J1)

Novus Biologicals | Catalog # NBP3-15914

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3T2J1 expressed in HEK293
View all ZBTB7A/Pokemon Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 450-550 of human ZBTB7A/Pokemon (O95365). NYDLKNHMRVHTGLRPYQCDSCCKTFVRSDHLHRHLKKDGCNGVPSRRGRKPRVRGGAPDPSPGATATPGAPAQPSSPDARRNGQEKHFKDEDEDEDVASP

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ZBTB7A/Pokemon Antibody (3T2J1)

Western Blot: ZBTB7A/Pokemon Antibody (3T2J1) [NBP3-15914]

Western Blot: ZBTB7A/Pokemon Antibody (3T2J1) [NBP3-15914]

Western Blot: ZBTB7A/Pokemon Antibody (3T2J1) [NBP3-15914] - Western blot analysis of extracts of various cell lines, using ZBTB7A/Pokemon Rabbit mAb (NBP3-15914) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry-Paraffin: ZBTB7A/Pokemon Antibody (3T2J1) [NBP3-15914]

Immunohistochemistry-Paraffin: ZBTB7A/Pokemon Antibody (3T2J1) [NBP3-15914]

Immunohistochemistry-Paraffin: ZBTB7A/Pokemon Antibody (3T2J1) [NBP3-15914] - Immunohistochemistry of paraffin-embedded human colon carcinoma using ZBTB7A/Pokemon Rabbit mAb (NBP3-15914) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ZBTB7A/Pokemon Antibody (3T2J1)

Immunocytochemistry/ Immunofluorescence: ZBTB7A/Pokemon Antibody (3T2J1) [ZBTB7A/Pokemon] -

Immunocytochemistry/ Immunofluorescence: ZBTB7A/Pokemon Antibody (3T2J1) [ZBTB7A/Pokemon] - Confocal imaging of U-2 OS cells using ZBTB7A/Pokemon Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
ZBTB7A/Pokemon Antibody (3T2J1)

ZBTB7A/Pokemon Antibody (3T2J1) [ZBTB7A/Pokemon] -

Confocal imaging of MCF7 cells using ZBTB7A/FBI-1/LRF Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with alpha -Tubulin Mouse mAb (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Applications for ZBTB7A/Pokemon Antibody (3T2J1)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL.

Immunocytochemistry/ Immunofluorescence

1:200 - 1:2000

Immunohistochemistry

1:100 - 1:1000

Immunohistochemistry-Paraffin

1:100 - 1:1000

Western Blot

1:1000 - 1:6000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ZBTB7A/Pokemon

Pokemon (POK Erythroid Myeloid Ontogenic factor) is part of the POK gene family that encodes proteins. It is an oncogene, but its role is unique in that it controls the activity of other oncogenes. POK proteins are critical in embryonic development, cellular differentiation, and oncogenesis.

Long Name

Zinc Finger and BTB Domain-Containing Protein 7A

Alternate Names

FBI-1, LRF, Pokemon, TIP21

Gene Symbol

ZBTB7A

Additional ZBTB7A/Pokemon Products

Product Documents for ZBTB7A/Pokemon Antibody (3T2J1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ZBTB7A/Pokemon Antibody (3T2J1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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