ZEB1 Antibody (2A8A6) - BSA Free

Novus Biologicals | Catalog # NBP2-23484

Novus Biologicals

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2A8A6

Format

BSA Free
View all ZEB1 Primary Antibodies »

Product Specifications

Immunogen

Partial recombinant human ZEB1 (between residues 800-1124) expressed in E. coli [Uniprot P37275]

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31331982).

Marker

Mesenchymal Cells Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for ZEB1 Antibody (2A8A6) - BSA Free

Western Blot: ZEB1 Antibody (2A8A6)BSA Free [NBP2-23484]

Western Blot: ZEB1 Antibody (2A8A6)BSA Free [NBP2-23484]

ZEB1-Antibody-2A8A6-Western-Blot-NBP2-23484-img0014.jpg
Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody (2A8A6) - BSA Free [NBP2-23484]

Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody (2A8A6) - BSA Free [NBP2-23484]

Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (2A8A6) [NBP2-23484] - ZEB1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Immunohistochemistry: ZEB1 Antibody (2A8A6) - BSA Free [NBP2-23484]

Immunohistochemistry: ZEB1 Antibody (2A8A6) - BSA Free [NBP2-23484]

Immunohistochemistry: ZEB1 Antibody (2A8A6) [NBP2-23484] - IHC analysis of paraffin-embedded human cervical cancer tissues with DAB staining.
Flow Cytometry: ZEB1 Antibody (2A8A6) - BSA Free [NBP2-23484]

Flow Cytometry: ZEB1 Antibody (2A8A6) - BSA Free [NBP2-23484]

Flow Cytometry: ZEB1 Antibody (2A8A6) [NBP2-23484] - An intracellular stain was performed on U-87 cells with ZEB1 [2A8A6] Antibody NBP2-23484AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.
Western Blot: ZEB1 Antibody (2A8A6)BSA Free [NBP2-23484]

Western Blot: ZEB1 Antibody (2A8A6)BSA Free [NBP2-23484]

Western Blot: ZEB1 Antibody (2A8A6) [NBP2-23484] - ZEB1 expression was assayed in lung adenocarcinoma cells after transfectionwith miR-33b or miR-NC, anti-miR-33b or anti-miR-NC in H1299 and A549 cells. Image submitted by verified customer review.
Immunohistochemistry: ZEB1 Antibody (2A8A6) - BSA Free [NBP2-23484]

Immunohistochemistry: ZEB1 Antibody (2A8A6) - BSA Free [NBP2-23484]

Immunohistochemistry: ZEB1 Antibody (2A8A6) [NBP2-23484] - IHC analysis of paraffin-embedded human recturm cancer tissues with DAB staining.
Flow Cytometry: ZEB1 Antibody (2A8A6) - BSA Free [NBP2-23484]

Flow Cytometry: ZEB1 Antibody (2A8A6) - BSA Free [NBP2-23484]

Flow Cytometry: ZEB1 Antibody (2A8A6) [NBP2-23484] - Flow cytometric analysis of Hela cells using ZEB1 mouse mAb (green) and negative control (red).
Flow Cytometry: ZEB1 Antibody (2A8A6) - BSA Free [NBP2-23484]

Flow Cytometry: ZEB1 Antibody (2A8A6) - BSA Free [NBP2-23484]

Flow Cytometry: ZEB1 Antibody (2A8A6) [NBP2-23484] - An intracellular stain was performed on with A549 cells with ZEB1 Antibody NBP2-23484PE and a a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.
Flow Cytometry: ZEB1 Antibody (2A8A6) - BSA Free [NBP2-23484]

Flow Cytometry: ZEB1 Antibody (2A8A6) - BSA Free [NBP2-23484]

Flow Cytometry: ZEB1 Antibody (2A8A6) [NBP2-23484] - An intracellular stain was performed on Jurkat with ZEB1 Antibody (2A8A6) NBP2-23484 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody (R&D Systems, F0102B)

Applications for ZEB1 Antibody (2A8A6) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1: 500 - 1:2000. Use reported by customer review and in scientific literature (PMID 29383119)
Application Notes
This antibody has had weak results in Western blot.

Reviewed Applications

Read 1 review rated 5 using NBP2-23484 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZEB1

ZEB1 (zinc-finger E-box-binding homeobox 1), also known as TCF8 (transcription factor 8), is a delta-EF1/ZFH-1 C2H2-type zinc finger that bears seven C2H2-type zinc fingers and one homeobox DNA-binding domain. ZEB1 is a transcriptional repressor of interleukin-2 (IL-2). Mutations in the ZEB1 gene are associated with posterior corneal dystrophy type 3 (PPC3) a rare disorder characterized by metaplasia and overgrowth of corneal endothelial cells. ZEB1 appears to be critical to epithelial to mesenchymal transitions (EMT) during development and cancer metastasis. As a transcription factor involved in EMT, ZEB1 promotes EMT by repressing genes associated with the epithelial phenotype and activates genes associated with the mesenchymal phenotype.

Long Name

Zinc Finger E-box Binding Homeobox 1

Alternate Names

AREB6, BZP, DELTAEF1, FECD6, NIL2A, PPCD3, TCF8, ZFHEP

Entrez Gene IDs

6935 (Human)

Gene Symbol

ZEB1

UniProt

P37275

Additional ZEB1 Products

Product Documents for ZEB1 Antibody (2A8A6) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ZEB1 Antibody (2A8A6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ZEB1 Antibody (2A8A6) - BSA Free

Customer Reviews for ZEB1 Antibody (2A8A6) - BSA Free (1)

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Customer Images

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  • ZEB1 Antibody (2A8A6)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: H1299 and A549 cells of lung adenocarcinoma cells
    Species: Human
    Verified Customer | Posted 09/14/2017
    ZEB1 expression was assayed in lung adenocarcinoma cells after transfection with miR-33b or miR-NC, anti-miR-33b or anti-miR-NC in H1299 and A549 cells.
    at a dilution of 1:800;secondary goat anti-mouse (1:5,000) immunoglobulin G.
    ZEB1 Antibody (2A8A6) - BSA Free NBP2-23484

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Protocols

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FAQs for ZEB1 Antibody (2A8A6) - BSA Free

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  • Q: In looking at the Zeb1 antibodies on your website, the molecular weights of Zeb1 detected by several antibodies are different. Why is this?

    A: ZEB1 is a rather large protein and undergoes a lot of post translational modifications such as phosphorylation and glycosylation (please see: UniProt P37275). The reason band patterns are different in the images may be due to sample type and how highly modified the ZEB1 protein is that the antibody is detecting. The more post translational modifications the higher you will detect the protein.

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