ZHX2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-82956

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ZHX2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PQEYDQLAAKTGLVRTEIVRWFKENRCLLKTGTVKWMEQYQHQPMADDHGYDAVARKATKPMAESPKNGGDVVPQYYKDPKKLCEEDLEKLVTRVKVGSEPAKDCLPAKPSEATSDRSEGSSRDGQGSDENEESSVVDYV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZHX2 Antibody - BSA Free

Western Blot: ZHX2 Antibody [NBP1-82956]

Western Blot: ZHX2 Antibody [NBP1-82956]

Western Blot: ZHX2 Antibody [NBP1-82956] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-651
Immunocytochemistry/ Immunofluorescence: ZHX2 Antibody [NBP1-82956]

Immunocytochemistry/ Immunofluorescence: ZHX2 Antibody [NBP1-82956]

Immunocytochemistry/Immunofluorescence: ZHX2 Antibody [NBP1-82956] - Staining of human cell line REH shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: ZHX2 Antibody [NBP1-82956]

Immunohistochemistry-Paraffin: ZHX2 Antibody [NBP1-82956]

Immunohistochemistry-Paraffin: ZHX2 Antibody [NBP1-82956] - Staining of human cerebral cortex.
Immunohistochemistry-Paraffin: ZHX2 Antibody [NBP1-82956]

Immunohistochemistry-Paraffin: ZHX2 Antibody [NBP1-82956]

Immunohistochemistry-Paraffin: ZHX2 Antibody [NBP1-82956] - Staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: ZHX2 Antibody [NBP1-82956]

Immunohistochemistry-Paraffin: ZHX2 Antibody [NBP1-82956]

Immunohistochemistry-Paraffin: ZHX2 Antibody [NBP1-82956] - Staining of human cerebral cortex, kidney, liver and lymph node using Anti-ZHX2 antibody NBP1-82956 (A) shows similar protein distribution across tissues to independent antibody NBP1-82955 (B).
Immunohistochemistry-Paraffin: ZHX2 Antibody [NBP1-82956]

Immunohistochemistry-Paraffin: ZHX2 Antibody [NBP1-82956]

Immunohistochemistry-Paraffin: ZHX2 Antibody [NBP1-82956] - Staining of human lymph node.
Immunohistochemistry-Paraffin: ZHX2 Antibody [NBP1-82956]

Immunohistochemistry-Paraffin: ZHX2 Antibody [NBP1-82956]

Immunohistochemistry-Paraffin: ZHX2 Antibody [NBP1-82956] - Staining of human kidney.
Immunohistochemistry-Paraffin: ZHX2 Antibody [NBP1-82956]

Immunohistochemistry-Paraffin: ZHX2 Antibody [NBP1-82956]

Immunohistochemistry-Paraffin: ZHX2 Antibody [NBP1-82956] - Staining of human liver.
ZHX2 Antibody - BSA Free Chromatin Immunoprecipitation ChIP: ZHX2 Antibody - BSA Free

Chromatin Immunoprecipitation ChIP: ZHX2 Antibody - BSA Free

ChIP-Exo-Seq composite graph for Anti-ZHX2 tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh's Lab at Cornell University.

Applications for ZHX2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZHX2

The members of the zinc fingers and homeoboxes gene family are nuclear homodimeric transcriptional repressors that interact with the A subunit of nuclear factor-Y (NF-YA) and contain two C2H2-type zinc fingers and five homeobox DNA-binding domains. This gene encodes member 2 of this gene family. In addition to forming homodimers, this protein heterodimerizes with member 1 of the zinc fingers and homeoboxes family.

Alternate Names

Zona pellucida glycoprotein 2, zona pellucida glycoprotein 2 (sperm receptor), zona pellucida glycoprotein ZP2, Zona pellucida protein A, zona pellucida sperm-binding protein 2, Zp-2, ZPA

Gene Symbol

ZHX2

Additional ZHX2 Products

Product Documents for ZHX2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ZHX2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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