![Western Blot: ZHX3 Antibody [NB100-68220]](https://resources.rndsystems.com/images/products/ZHX3-Antibody-Western-Blot-NB100-68220-img0005.jpg "Western Blot: ZHX3 Antibody [NB100-68220]")
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Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 906 and 956 of human zinc fingers and homeoboxes 3 using the numbering given in entry NP_055850.1 (GeneID 23051).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ZHX3 Antibody
![Immunocytochemistry/ Immunofluorescence: ZHX3 Antibody [NB100-68220]](https://resources.rndsystems.com/images/products/ZHX3-Antibody-Immunocytochemistry-Immunofluorescence-NB100-68220-img0004.jpg "Immunocytochemistry/ Immunofluorescence: ZHX3 Antibody [NB100-68220]")
Immunocytochemistry/ Immunofluorescence: ZHX3 Antibody [NB100-68220]
Immunocytochemistry/Immunofluorescence: ZHX3 Antibody [NB100-68220] - FFPE section of human prostate carcinoma. Affinity purified rabbit anti-ZHX3 used at a dilution of 1:100. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte PlusTM 555 used at a dilution of 1:100.![Immunohistochemistry: ZHX3 Antibody [NB100-68220]](https://resources.rndsystems.com/images/products/ZHX3-Antibody-Immunohistochemistry-NB100-68220-img0006.jpg "Immunohistochemistry: ZHX3 Antibody [NB100-68220]")
Immunohistochemistry: ZHX3 Antibody [NB100-68220]
Immunohistochemistry: ZHX3 Antibody [NB100-68220] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- ZHX3 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB![Immunohistochemistry-Paraffin: ZHX3 Antibody [NB100-68220]](https://resources.rndsystems.com/images/products/ZHX3-Antibody-Immunohistochemistry-Paraffin-NB100-68220-img0001.jpg "Immunohistochemistry-Paraffin: ZHX3 Antibody [NB100-68220]")
Immunohistochemistry-Paraffin: ZHX3 Antibody [NB100-68220]
Immunohistochemistry-Paraffin: ZHX3 Antibody [NB100-68220] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-ZHX3 used at a dilution of 1:500. Detection: DAB staining using Immunohistochemistry Accessory Kit![Immunohistochemistry-Paraffin: ZHX3 Antibody [NB100-68220]](https://resources.rndsystems.com/images/products/ZHX3-Antibody-Immunohistochemistry-Paraffin-NB100-68220-img0002.jpg "Immunohistochemistry-Paraffin: ZHX3 Antibody [NB100-68220]")
Immunohistochemistry-Paraffin: ZHX3 Antibody [NB100-68220]
Immunohistochemistry-Paraffin: ZHX3 Antibody [NB100-68220] - FFPE section of human prostate carcinoma. Affinity purified rabbit anti-ZHX3 used at a dilution of 1:500. Detection: DAB staining using Immunohistochemistry Accessory KitApplications for ZHX3 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50- 1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: ZHX3
Alternate Names
FLJ93221, SP38, zona pellucida binding protein, zona pellucida-binding protein 1, ZPBP1zona pellucida binding protein 1
Entrez Gene IDs
23051 (Human)
Gene Symbol
ZHX3
UniProt
Additional ZHX3 Products
Product Documents for ZHX3 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ZHX3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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