ZNF667 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-77357

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human MIPU1. The immunogen is located within the first 50 amino acids of MIPU1. Amino Acid Squence: SQEEWEWLSPIQKDLYE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZNF667 Antibody - BSA Free

Western Blot: ZNF667 AntibodyBSA Free [NBP1-77357]

Western Blot: ZNF667 AntibodyBSA Free [NBP1-77357]

Western Blot: ZNF667 Antibody [NBP1-77357] - Analysis in human heart tissue lysate with antibody at 1 ug/mL.
ZNF667 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: ZNF667 Antibody - BSA Free [NBP1-77357] -

Immunocytochemistry/ Immunofluorescence: ZNF667 Antibody - BSA Free [NBP1-77357] - Immunofluorescence of MIPU in human brain tissue with MIPU antibody at 20 u/mL.
ZNF667 Antibody - BSA Free

Immunohistochemistry: ZNF667 Antibody - BSA Free [NBP1-77357] -

Immunohistochemistry: ZNF667 Antibody - BSA Free [NBP1-77357] - Immunohistochemistry of ZNF667 in human brain tissue with ZNF667 antibody at 2.5 ug/mL.
ZNF667 Antibody - BSA Free

Western Blot: ZNF667 Antibody - BSA Free [NBP1-77357] -

ZNF667 and ZNF667-AS1 as novel markers for prognosis in PDAC.a Immunohistochemistry of ZNF667 shows variations of abundance across patients using the tissue microarray analysis. b Overall survival and progression-free survival of self-collected PDAC patients grouped by the abundance of ZNF667. c Relative abundance of ZNF667 and ZNF667-AS1 to ACTB mRNA in normal pancreas cells (HPDE6-C7), human embryonic kidney cells (HEK293T), liver metastasis pancreas cancer cells (L3.7), and the pancreas cancer cells detected by RT-qPCR. All pancreas cancer cells showed a decreased level of ZNF667 and ZNF667-AS1. *P < 0.05; **P < 0.01. d Immunoblot showing the decreased abundance of ZNF667 in multiple pancreas cancer cell lines. The abundance was calculated at the bottom. e, f Validation of ZNF667 and ZNF667-AS1 overexpression (OE) in two pancreas cancer cell lines. **P < 0.01; ****P < 0.0001. g Immunoblot showing the abundance of ZNF667 in PANC-1 and SW1990 cells before and after overexpression. h, i Proliferation evaluation (CCK8-positive cell ratio) after ZNF667 and ZNF667-AS1 overexpression in two pancreas cancer cell lines. n = 3 for each time point. **P < 0.001; ****P < 0.00001. j Evaluation of cellular apoptosis after ZNF667-AS1 overexpression in two pancreas cancer cell lines. n.s., no significance. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35165277), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
ZNF667 Antibody - BSA Free

Western Blot: ZNF667 Antibody - BSA Free [NBP1-77357] -

ZNF667 and ZNF667-AS1 as novel markers for prognosis in PDAC.a Immunohistochemistry of ZNF667 shows variations of abundance across patients using the tissue microarray analysis. b Overall survival and progression-free survival of self-collected PDAC patients grouped by the abundance of ZNF667. c Relative abundance of ZNF667 and ZNF667-AS1 to ACTB mRNA in normal pancreas cells (HPDE6-C7), human embryonic kidney cells (HEK293T), liver metastasis pancreas cancer cells (L3.7), and the pancreas cancer cells detected by RT-qPCR. All pancreas cancer cells showed a decreased level of ZNF667 and ZNF667-AS1. *P < 0.05; **P < 0.01. d Immunoblot showing the decreased abundance of ZNF667 in multiple pancreas cancer cell lines. The abundance was calculated at the bottom. e, f Validation of ZNF667 and ZNF667-AS1 overexpression (OE) in two pancreas cancer cell lines. **P < 0.01; ****P < 0.0001. g Immunoblot showing the abundance of ZNF667 in PANC-1 and SW1990 cells before and after overexpression. h, i Proliferation evaluation (CCK8-positive cell ratio) after ZNF667 and ZNF667-AS1 overexpression in two pancreas cancer cell lines. n = 3 for each time point. **P < 0.001; ****P < 0.00001. j Evaluation of cellular apoptosis after ZNF667-AS1 overexpression in two pancreas cancer cell lines. n.s., no significance. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35165277), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for ZNF667 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZNF667

Mipu1, also known as zinc finger protein 667 or ZNF667, encodes a nuclear-localized protein containing 14 carboxy-terminal zinc finger motifs and an amino-terminal KRAB domain. This protein is highly expressed in heart and brain and is upregulated in rat heart after a transient ischemia-reperfusion procedure. Overexpression experiments suggest that Mipu1 suppresses the transcriptional activities of AP-1 and SRE in the MAPK signaling pathway and thus may play a role in the pathogenesis of cardiac and vascular disease. At least four isoforms of MIPU1 are known to exist.

Alternate Names

DKFZp686O111, FLJ14011, FLJ45518, MIPU1, myocardial ischemic preconditioning upregulated 1 ortholog, zinc finger protein 667

Gene Symbol

ZNF667

Additional ZNF667 Products

Product Documents for ZNF667 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ZNF667 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ZNF667 Antibody - BSA Free

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Protocols

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