AK3 Antibody (SJB3-36) - BSA Free
Novus Biologicals | Catalog # NBP1-04261
![Western Blot: AK3 Antibody (SJB3-36)BSA Free [NBP1-04261]](https://resources.rndsystems.com/images/products/AK3-Antibody-SJB3-36-Western-Blot-NBP1-04261-img0007.jpg "Western Blot: AK3 Antibody (SJB3-36)BSA Free [NBP1-04261]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SJB3-36
Format
BSA Free
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Product Specifications
Immunogen
Recombinant human AK3 (adenylaste kinase isozyme 3) purified from E. coli
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for AK3 Antibody (SJB3-36) - BSA Free
Western Blot: AK3 Antibody (SJB3-36)BSA Free [NBP1-04261]
Western Blot: AK3 Antibody (SJB3-36) [NBP1-04261] - The recombinant protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AK3 antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Recombinant Human AK1 Lane 2. : Recombinant Human AK2 Lane 3. : Recombinant Human AK3![Immunocytochemistry/ Immunofluorescence: AK3 Antibody (SJB3-36) - BSA Free [NBP1-04261]](https://resources.rndsystems.com/images/products/AK3-Antibody-SJB3-36-Immunocytochemistry-Immunofluorescence-NBP1-04261-img0004.jpg "Immunocytochemistry/ Immunofluorescence: AK3 Antibody (SJB3-36) - BSA Free [NBP1-04261]")
Immunocytochemistry/ Immunofluorescence: AK3 Antibody (SJB3-36) - BSA Free [NBP1-04261]
Immunocytochemistry/Immunofluorescence: AK3 Antibody (SJB3-36) [NBP1-04261] - Analysis of AK3 in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AK3 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).![Immunohistochemistry-Paraffin: AK3 Antibody (SJB3-36) - BSA Free [NBP1-04261]](https://resources.rndsystems.com/images/products/AK3-Antibody-SJB3-36-Immunohistochemistry-Paraffin-NBP1-04261-img0001.jpg "Immunohistochemistry-Paraffin: AK3 Antibody (SJB3-36) - BSA Free [NBP1-04261]")
Immunohistochemistry-Paraffin: AK3 Antibody (SJB3-36) - BSA Free [NBP1-04261]
Immunohistochemistry-Paraffin: AK3 Antibody (SJB3-36) [NBP1-04261] - Human liver tissue was incubated with anti-human AK3 (1:100) for 2 hours at room temperature. Slide was then washed in PBS, and was incubated in avidin biosystem anti-rabbit labeled polymer for 30 min at RT. Enzyme detection was performed with DAB chromo-gen.![Flow Cytometry: AK3 Antibody (SJB3-36) - BSA Free [NBP1-04261]](https://resources.rndsystems.com/images/products/AK3-Antibody-SJB3-36-Flow-Cytometry-NBP1-04261-img0003.jpg "Flow Cytometry: AK3 Antibody (SJB3-36) - BSA Free [NBP1-04261]")
Flow Cytometry: AK3 Antibody (SJB3-36) - BSA Free [NBP1-04261]
Flow Cytometry: AK3 Antibody (SJB3-36) [NBP1-04261] - Analysis of AK3 in jurkat cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).![Western Blot: AK3 Antibody (SJB3-36)BSA Free [NBP1-04261]](https://resources.rndsystems.com/images/products/AK3-Antibody-SJB3-36-Western-Blot-NBP1-04261-img0005.jpg "Western Blot: AK3 Antibody (SJB3-36)BSA Free [NBP1-04261]")
Western Blot: AK3 Antibody (SJB3-36)BSA Free [NBP1-04261]
Western Blot: AK3 Antibody (SJB3-36) [NBP1-04261] - The lysate (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human AK3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Mouse liver tissue lysate![Western Blot: AK3 Antibody (SJB3-36)BSA Free [NBP1-04261]](https://resources.rndsystems.com/images/products/AK3-Antibody-SJB3-36-Western-Blot-NBP1-04261-img0006.jpg "Western Blot: AK3 Antibody (SJB3-36)BSA Free [NBP1-04261]")
Western Blot: AK3 Antibody (SJB3-36)BSA Free [NBP1-04261]
Western Blot: AK3 Antibody (SJB3-36) [NBP1-04261] - The lysate (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human AK3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Hep3B cell lysate Lane 2. : 293T cell lysate Lane 3. : Mouse kidney tissue lysate![AK3 Antibody (SJB3-36) - BSA Free Western Blot: AK3 Antibody (SJB3-36) [NBP1-04261]](https://resources.rndsystems.com/images/products/nbp1-04261_mouse-ak3-mab-sjb3-36-bsa-free-26420231611592.jpg "Western Blot: AK3 Antibody (SJB3-36) [NBP1-04261]")
Western Blot: AK3 Antibody (SJB3-36) [NBP1-04261]
The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with NBP1-04261 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HepG2 cell lysate. Lane 2.: Lncap cell lysateApplications for AK3 Antibody (SJB3-36) - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:100
Western Blot
1:500-1:2000
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 10% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: AK3
Long Name
GTP:AMP phosphotransferase AK3, mitochondrial
Alternate Names
Adenylate kinase 3, AK 3, AK3L1, AK6, AKL3L, EC 2.7.4.10
Gene Symbol
AK3
UniProt
Additional AK3 Products
Product Documents for AK3 Antibody (SJB3-36) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for AK3 Antibody (SJB3-36) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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