Aminoacylase/ACY1 Antibody (OTI1D4)

Novus Biologicals | Catalog # NBP2-00970

Novus Biologicals
Discontinued Product
NBP2-00970 has been discontinued. View all Aminoacylase/ACY1 products.

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1D4
View all Aminoacylase/ACY1 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human ACY1(NP_000657) produced in HEK293 cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

45.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Aminoacylase/ACY1 Antibody (OTI1D4)

Western Blot: Aminoacylase/ACY1 Antibody (OTI1D4) [NBP2-00970]

Western Blot: Aminoacylase/ACY1 Antibody (OTI1D4) [NBP2-00970]

Western Blot: Aminoacylase/ACY1 Antibody (OTI1D4) [NBP2-00970] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Aminoacylase 1 monoclonal antibody.
Immunohistochemistry-Paraffin: Aminoacylase/ACY1 Antibody (OTI1D4) [NBP2-00970]

Immunohistochemistry-Paraffin: Aminoacylase/ACY1 Antibody (OTI1D4) [NBP2-00970]

Immunohistochemistry-Paraffin: Aminoacylase/ACY1 Antibody (OTI1D4) [NBP2-00970] - Staining of paraffin-embedded Human prostate tissue using anti-Aminoacylase 1 mouse monoclonal antibody.
Flow Cytometry: Aminoacylase/ACY1 Antibody (OTI1D4) [NBP2-00970]

Flow Cytometry: Aminoacylase/ACY1 Antibody (OTI1D4) [NBP2-00970]

Flow Cytometry: Aminoacylase/ACY1 Antibody (OTI1D4) [NBP2-00970] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Aminoacylase 1 antibody, and then analyzed by flow cytometry.
Western Blot: Aminoacylase/ACY1 Antibody (OTI1D4) [NBP2-00970]

Western Blot: Aminoacylase/ACY1 Antibody (OTI1D4) [NBP2-00970]

Western Blot: Aminoacylase/ACY1 Antibody (OTI1D4) [NBP2-00970] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Aminoacylase 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Aminoacylase 1.
Immunohistochemistry-Paraffin: Aminoacylase/ACY1 Antibody (OTI1D4) [NBP2-00970]

Immunohistochemistry-Paraffin: Aminoacylase/ACY1 Antibody (OTI1D4) [NBP2-00970]

Immunohistochemistry-Paraffin: Aminoacylase/ACY1 Antibody (OTI1D4) [NBP2-00970] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Aminoacylase 1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Aminoacylase/ACY1 Antibody (OTI1D4) [NBP2-00970]

Immunohistochemistry-Paraffin: Aminoacylase/ACY1 Antibody (OTI1D4) [NBP2-00970]

Immunohistochemistry-Paraffin: Aminoacylase/ACY1 Antibody (OTI1D4) [NBP2-00970] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Aminoacylase 1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Aminoacylase/ACY1 Antibody (OTI1D4) [NBP2-00970]

Immunohistochemistry-Paraffin: Aminoacylase/ACY1 Antibody (OTI1D4) [NBP2-00970]

Immunohistochemistry-Paraffin: Aminoacylase/ACY1 Antibody (OTI1D4) [NBP2-00970] - Staining of paraffin-embedded Human Kidney tissue using anti-Aminoacylase 1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Aminoacylase/ACY1 Antibody (OTI1D4) [NBP2-00970]

Immunohistochemistry-Paraffin: Aminoacylase/ACY1 Antibody (OTI1D4) [NBP2-00970]

Immunohistochemistry-Paraffin: Aminoacylase/ACY1 Antibody (OTI1D4) [NBP2-00970] - Staining of paraffin-embedded Human liver tissue using anti-Aminoacylase 1 mouse monoclonal antibody.

Applications for Aminoacylase/ACY1 Antibody (OTI1D4)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

Flow Cytometry Panel Builder

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Aminoacylase/ACY1

This gene encodes a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and an acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. This gene is located on chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and this enzyme is the first member of a new family of zinc-binding enzymes. Mutations in this gene cause aminoacylase-1 deficiency, a metabolic disorder characterized by central nervous system defects and increased urinary excretion of N-acetylated amino acids. Alternative splicing of this gene results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ABHD14A (abhydrolase domain containing 14A) gene, as represented in GeneID:100526760. A related pseudogene has been identified on chromosome 18. [provided by RefSeq].

Alternate Names

ACY1

Entrez Gene IDs

95 (Human)

Gene Symbol

ACY1

Additional Aminoacylase/ACY1 Products

Product Documents for Aminoacylase/ACY1 Antibody (OTI1D4)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Aminoacylase/ACY1 Antibody (OTI1D4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Metalloproteases and Regulators

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Protocols

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