Argininosuccinate Synthase Antibody

Novus Biologicals | Catalog # NBP1-00153

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat, Bovine

Cited:

Rat

Predicted:

Canine (100%), Mouse (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Argininosuccinate Synthase Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-ENPKNQAPPGLYTKTQD corresponding to internal region according to NP_446464.1.

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 18948083); Rat reactivity reported in scientific literature (PMID: 24113767).

Specificity

The variants represent identical protein (NP_000041.2 and NP_446464.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Argininosuccinate Synthase Antibody

Western Blot: Argininosuccinate Synthase Antibody [NBP1-00153]

Western Blot: Argininosuccinate Synthase Antibody [NBP1-00153]

Western Blot: Argininosuccinate Synthase Antibody [NBP1-00153] - Staining of Human Kidney (A) Mouse Liver (B) with antibody at 0.01 ug/mL and Rat Kidney (C) lysate with antibody at 0.03 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Argininosuccinate Synthase Antibody [NBP1-00153]

Immunocytochemistry/ Immunofluorescence: Argininosuccinate Synthase Antibody [NBP1-00153]

Immunocytochemistry/Immunofluorescence: Argininosuccinate Synthase Antibody [NBP1-00153] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-00153]

Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-00153]

Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-00153] - (2.5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: Argininosuccinate Synthase Antibody [NBP1-00153]

Flow Cytometry: Argininosuccinate Synthase Antibody [NBP1-00153]

Flow Cytometry: Argininosuccinate Synthase Antibody [NBP1-00153] - Analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Western Blot: Argininosuccinate Synthase Antibody [NBP1-00153]

Western Blot: Argininosuccinate Synthase Antibody [NBP1-00153]

Western Blot: Argininosuccinate Synthase Antibody [NBP1-00153] - Staining of A431 (A) and (1ug/ml) NIH3T3(B) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-00153]

Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-00153]

Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-00153] - (2.5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Argininosuccinate Synthase Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry

2 - 4 ug/mL

Immunohistochemistry-Paraffin

2 - 4 ug/mL

Peptide ELISA

Detection limit 1:64000

Western Blot

0.03 - 1 ug/mL
Application Notes
IP successful usage from BAEC cells is reported in scientific literature (PMID: 18948083).
WB: Approx. 45 kDa band observed in human kidney lysates (calculated MW of 46.5 kDa band according to NP_000041.2 and NP_446464.1). IHC-P: Human kidney shows textured cytoplasm staining in PCT.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Argininosuccinate Synthase

The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of ASS cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Alternate Names

argininosuccinate synthase, argininosuccinate synthase 1, argininosuccinate synthetase, argininosuccinate synthetase 1, ASSCTLN1, citrulline-aspartate ligase, Citrulline--aspartate ligase, EC 6.3.4.5

Entrez Gene IDs

445 (Human); 11898 (Mouse); 25698 (Rat)

Gene Symbol

ASS1

UniProt

NP_000041.2 (Human)

Additional Argininosuccinate Synthase Products

Product Documents for Argininosuccinate Synthase Antibody

Certificate of Analysis

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Product Specific Notices for Argininosuccinate Synthase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Argininosuccinate Synthase Antibody

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Protocols

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